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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CRYZ Polyclonal antibody (31629-1-AP)

    WB analysis using 31629-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31629-1-AP (CRYZ antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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CRYZ Polyclonal antibody

CRYZ Polyclonal Antibody for WB, ELISA
Cat No. 31629-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

QOR

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse kidney tissue, mouse liver tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31629-1-AP targets CRYZ in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CRYZ fusion protein Ag35066 相同性解析による交差性が予測される生物種
Full Name crystallin, zeta (quinone reductase)
Calculated molecular weight 35 kDa
Observed molecular weight 35 kDa
GenBank accession numberBC039578
Gene Symbol CRYZ
Gene ID (NCBI) 1429
RRIDAB_3670055
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ08257
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CRYZ (Zeta-crystallin) also called quinone oxidoreductase and NADPH:quinone reductase, and it does not have alcohol dehydrogenase activity. It binds NADP and acts through a one-electron transfer process. Orthoquinones, such as 1,2-naphthoquinone or 9,10-phenanthrenequinone, are the best substrates (in vitro) for CRYZ. It may act in the detoxification of xenobiotics. CRYZ interacts with (AU)-rich elements (ARE) in the 3'-UTR of target mRNA species and enhances the stability of mRNA coding for BCL2. NADPH binding interferes with mRNA binding (PMID: 17497241; 20103721).

Protocols

Product Specific Protocols
WB protocol for CRYZ antibody 31629-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols