Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse kidney tissue, rat kidney tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
33679-1-AP targets CRYL1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag38269 Product name: Recombinant human CRYL1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-319 aa of NM_015974.2 Sequence: MASSAAGCVVIVGSGVIGRSWAMLFASGGFQVKLYDIEQQQIRNALENIRKEMKLLEQAGSLKGSLSVEEQLSLISGCPNIQEAVEGAMHIQECVPEDLELKKKIFAQLDSIIDDRVILSSSTSCLMPSKLFAGLVHVKQCIVAHPVNPPYYIPLVELVPHPETAPTTVDRTHALMKKIGQCPMRVQKEVAGFVLNRLQYAIISEAWRLVEEGIVSPSDLDLVMSEGLGMRYAFIGPLETMHLNAEGMLSYCDRYSEGIKHVLQTFGPIPEFSRATAEKVNQDMCMKVPDDPEHLAARRQWRDECLMRLAKLKSQVQPQ 相同性解析による交差性が予測される生物種 |
| Full Name | crystallin, lambda 1 |
| Calculated molecular weight | 35kDa，319aa |
| Observed molecular weight | 35 kDa |
| GenBank accession number | NM_015974.2 |
| Gene Symbol | CRYL1 |
| Gene ID (NCBI) | 51084 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q9Y2S2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CRYL1 (Crystallin Lambda 1) is a ubiquitously-expressed, dual-function protein that was first isolated from rabbit lens tissue. Human CRYL1 is encoded at chromosomal locus 13q12.11, contains two HCDH (3-hydroxyacyl-CoA dehydrogenase) domains, and is most abundant in liver and kidney, with only trace expression in peripheral-blood leukocytes and thymus. Further research showed that CRYL1 was expressed at lower levels in HCC tumors than in adjacent tissues, and this reduced expression was associated with advanced tumor staging, larger tumors, and decreased patient disease-free survival.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CRYL1 antibody 33679-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |