Validation Data Gallery
Tested Applications
| Positive WB detected in | rat eye tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:20000-1:100000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16542-1-AP targets CRYGS in WB, ELISA applications and shows reactivity with human, rat samples.
| Tested Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CRYGS fusion protein Ag9868 相同性解析による交差性が予測される生物種 |
| Full Name | crystallin, gamma S |
| Calculated molecular weight | 178 aa, 21 kDa |
| Observed molecular weight | 21 kDa |
| GenBank accession number | BC070241 |
| Gene Symbol | CRYGS |
| Gene ID (NCBI) | 1427 |
| RRID | AB_3669249 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P22914 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CRYGS (Gamma-S crystallin), also called CRYG8, belongs to the gamma-crystallins (PMID: 7733876; 23199295). Three major classes of crystallins, alpha, beta, and gamma, are common to the eye lens throughout all vertebrates. The beta and gamma classes are made up of homologous proteins and constitute the beta/gamma crystallin superfamily. CRYGS is expressed late but abundantly in the ocular lens (PMID: 1445197). It is also expressed outside the lens, particularly in the mature retina and cornea. In contrast to the beta-crystallins which associate in various combinations to form low or high molecular weight aggregates, CRYGS is, like the other gamma-crystallins, a monomeric protein (PMID: 16141006).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CRYGS antibody 16542-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |