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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CRYBB2 Monoclonal antibody (68481-1-Ig)

    WB analysis using 68481-1-Ig (same clone as 68481-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68481-1-Ig (CRYBB2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68481-1-PBS in a different storage buffer formulation.

CRYBB2 Monoclonal antibody, PBS Only

CRYBB2 Monoclonal Antibody for WB, Indirect ELISA
Cat No. 68481-1-PBS
Clone No.2E5E7

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, rabbit

Applications

WB, Indirect ELISA

Synonyms

2E5E7, Beta-B2 crystallin, Beta-crystallin B2, Beta-crystallin Bp, CCA2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68481-1-PBS targets CRYBB2 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat, rabbit samples.

Tested Reactivity human, mouse, rat, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag12916

Product name: Recombinant human CRYBB2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-205 aa of BC069535

Sequence: MASDHQTQAGKPQSLNPKIIIFEQENFQGHSHELNGPCPNLKETGVEKAGSVLVQAGPWVGYEQANCKGEQFVFEKGEYPRWDSWTSSRRTDSLSSLRPIKVDSQEHKIILYENPNFTGKKMEIIDDDVPSFHAHGYQEKVSSVRVQSGTWVGYQYPGYRGLQYLLEKGDYKDSSDFGAPHPQVQSVRRIRDMQWHQRGAFHPSN

相同性解析による交差性が予測される生物種
Full Name crystallin, beta B2
Calculated molecular weight 205 aa, 23 kDa
Observed molecular weight 24 kDa
GenBank accession numberBC069535
Gene Symbol CRYBB2
Gene ID (NCBI) 1415
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP43320
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.