Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse eye tissue, mouse brain tissue, fetal human brain tissue, rat eye tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
Product Information
18154-1-AP targets CRYBB2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12906 Product name: Recombinant human CRYBB2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-205 aa of BC069535 Sequence: MASDHQTQAGKPQSLNPKIIIFEQENFQGHSHELNGPCPNLKETGVEKAGSVLVQAGPWVGYEQANCKGEQFVFEKGEYPRWDSWTSSRRTDSLSSLRPIKVDSQEHKIILYENPNFTGKKMEIIDDDVPSFHAHGYQEKVSSVRVQSGTWVGYQYPGYRGLQYLLEKGDYKDSSDFGAPHPQVQSVRRIRDMQWHQRGAFHPSN 相同性解析による交差性が予測される生物種 |
| Full Name | crystallin, beta B2 |
| Calculated molecular weight | 205 aa, 23 kDa |
| Observed molecular weight | 24 kDa |
| GenBank accession number | BC069535 |
| Gene Symbol | CRYBB2 |
| Gene ID (NCBI) | 1415 |
| RRID | AB_2878510 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P43320 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CRYBB2 antibody 18154-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cells Effect of a Lens Protein in Low-Temperature Culture of Novel Immortalized Human Lens Epithelial Cells (iHLEC-NY2). | ||
Invest Ophthalmol Vis Sci Relationship Between the Altered Expression and Epigenetics of GSTM3 and Age-Related Cataract. | ||
FASEB J Knockout of miR-184 in zebrafish leads to ocular abnormalities by elevating p21 levels |
