Validation Data Gallery
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Tested Applications
| Positive WB detected in | rat eye tissue, C6 cells, mouse eye tissue, rabbit eye tissue |
| Positive IF/ICC detected in | hTERT-RPE1 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:300-1:1200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
60273-1-Ig targets CRYBB1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, rabbit samples.
| Tested Reactivity | human, mouse, rat, rabbit |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4722 Product name: Recombinant human CRYBB1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-252 aa of BC036790 Sequence: MSQAAKASASATVAVNPGPDTKGKGAPPAGTSPSPGTTLAPTTVPITSAKAAELPPGNYRLVVFELENFQGRRAEFSGECSNLADRGFDRVRSIIVSAGPWVAFEQSNFRGEMFILEKGEYPRWNTWSSSYRSDRLMSFRPIKMDAQEHKISLFEGANFKGNTIEIQGDDAPSLWVYGFSDRVGSVKVSSGTWVGYQYPGYRGYQYLLEPGDFRHWNEWGAFQPQMQSLRRLRDKQWHLEGSFPVLATEPPK 相同性解析による交差性が予測される生物種 |
| Full Name | crystallin, beta B1 |
| Calculated molecular weight | 252 aa, 28 kDa |
| Observed molecular weight | 30 kDa |
| GenBank accession number | BC036790 |
| Gene Symbol | CRYBB1 |
| Gene ID (NCBI) | 1414 |
| RRID | AB_2881393 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P53674 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Mutation in CRYBB1 is associated with congenital Cataract-Microcornea Syndrome.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CRYBB1 antibody 60273-1-Ig | Download protocol |
| WB protocol for CRYBB1 antibody 60273-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
