Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse eye tissue, rat eye tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:20000-1:100000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
33621-1-AP targets CRYBA4 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag39089 Product name: Recombinant human CRYBA4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-150 aa of BC069404 Sequence: MTLQCTKSAGPWKMVVWDEDGFQGRRHEFTAECPSVLELGFETVRSLKVLSGAWVGFEHAGFQGQQYILERGEYPSWDAWGGNTAYPAERLTSFRPAACANHRDSRLTIFEQENFLGKKGELSDDYPSLQAMGWEGNEVGSFHVHSGAWV 相同性解析による交差性が予測される生物種 |
| Full Name | crystallin, beta A4 |
| Calculated molecular weight | 196 aa, 22 kDa |
| Observed molecular weight | 22 kDa |
| GenBank accession number | BC069404 |
| Gene Symbol | CRYBA4 |
| Gene ID (NCBI) | 1413 |
| RRID | AB_3743031 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | P53673 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CRYBA4, also called βA4-crystallin, is the dominant structural components of the vertebrate eye lens (PMID: 16960806). Missense mutations of CRYBA4 (e.g., p.R95W, p.V150D) predicted to be highly pathogenic are linked with autosomal-dominant congenital cataract, indicating that both crystallin aggregation and non-lens signaling functions merit therapeutic targeting (PMID: 31935276).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CRYBA4 antibody 33621-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |