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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CRTAM Polyclonal antibody (16627-1-AP)

    WB analysis using 16627-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16627-1-AP (CRTAM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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CRTAM/CD355 Polyclonal antibody

CRTAM/CD355 Polyclonal Antibody for WB, ELISA
Cat No. 16627-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, ELISA

Synonyms

CRTAM, Cytotoxic and regulatory T-cell molecule, Class-I MHC-restricted T-cell-associated molecule, CD355

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse brain tissue, mouse thymus tissue, rat brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16627-1-AP targets CRTAM/CD355 in WB, ELISA applications and shows reactivity with mouse, rat samples.

Tested Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CRTAM/CD355 fusion protein Ag9956 相同性解析による交差性が予測される生物種
Full Name cytotoxic and regulatory T cell molecule
Calculated molecular weight 393 aa, 45 kDa
Observed molecular weight70 kDa
GenBank accession numberBC070266
Gene Symbol CRTAM
Gene ID (NCBI) 56253
RRIDAB_3085502
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95727
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cytotoxic and regulatory T cell molecule (CRTAM, also known as CD355) is an immunoglobulin-superfamily transmembrane protein with V and C1-like Ig domains, which is involved in epithelial cell adhesion (PMID: 18329370; 20556794). CRTAM is critical to instruct the differentiation of CD4+ cytotoxic T cells (CTL) through the induction of eomesodermin and CTL-related genes (PMID: 26694968). It also regulates CD8+ T cell retention within the lymph node and eventually effector function (PMID: 19752223). The predicted molecular weight of CRTAM is 43 kDa. Due to glycosylation, the observed molecular weight of CRTAM can be apparent at ~80 kDa (PMID: 16300832).

Protocols

Product Specific Protocols
WB protocol for CRTAM/CD355 antibody 16627-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols