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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CRTAC1 Polyclonal antibody (30251-1-AP)

    WB analysis using 30251-1-AP (same clone as 30251-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30251-1-AP (CRTAC1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30251-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CRTAC1 Polyclonal antibody (30251-1-AP)

    WB analysis using 30251-1-AP (same clone as 30251-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30251-1-AP (CRTAC1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30251-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HCT 116 cells using CRTAC1 Polyclonal antibody (30251-1-AP)

    WB analysis of HCT 116 using 30251-1-AP (same clone as 30251-1-PBS)

    WB result of CRTAC1 antibody (30251-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-CRTAC1 transfected HCT 116 cells. This data was developed using the same antibody clone with 30251-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CRTAC1 Polyclonal antibody (30251-1-AP)

    IHC staining of mouse brain using 30251-1-AP (same clone as 30251-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 30251-1-AP (CRTAC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30251-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using CRTAC1 Polyclonal antibody (30251-1-AP)

    IHC staining of rat brain using 30251-1-AP (same clone as 30251-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 30251-1-AP (CRTAC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30251-1-PBS in a different storage buffer formulation.

CRTAC1 Polyclonal antibody, PBS Only

CRTAC1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 30251-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

68 kDa chondrocyte-expressed protein, ASPIC, ASPIC1, Cartilage acidic protein 1, CEP 68

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

30251-1-PBS targets CRTAC1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag32963

Product name: Recombinant human CRTAC1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 198-332 aa of BC034245

Sequence: VVTDFDGDGMLDLILSHGESMAQPLSVFRGNQGFNNNWLRVVPRTRFGAFARGAKVVLYTKKSGAHLRIIDGGSGYLCEMEPVAHFGLGKDEASSVEVTWPDGKMVSRNVASGEMNSVLEILYPRDEDTLQDPAP

相同性解析による交差性が予測される生物種
Full Name cartilage acidic protein 1
Calculated molecular weight 661 aa, 71 kDa
Observed molecular weight 71-100 kDa
GenBank accession numberBC034245
Gene Symbol CRTAC1
Gene ID (NCBI) 55118
RRIDAB_3086281
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NQ79
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CRTAC1 (Cartilage acidic protein 1) is also named as ASPIC and 68 kDa chondrocyte-expressed protein (CEP-68). Cartilage acidic protein 1 (CRTAC1), a novel human marker which allowed discrimination of human chondrocytes from osteoblasts and mesenchymal stem cells (PMID: 17074475). CRTAC1 is a glycosylated calcium-binding extracellular matrix protein (PMID: 37796703). CRTAC1 acquired an alternate last exon from the tail-to-tail oriented neighbouring gene in humans resulting in the glycosylated isoform CRTAC1-A which represents a new extracellular matrix molecule of articular cartilage (PMID: 17074475). CRTAC1 was downregulated in bladder cancer tissues and cells (PMID: 34818994).

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