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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CRTAC1 Polyclonal antibody

CRTAC1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13001-1-AP

Synonyms

ASPIC, ASPIC1, cartilage acidic protein 1, CEP 68, CEP68, CRTAC1

Validation Data Gallery

Applications:
  • WB analysis of human brain using 13001-1-AP

    WB analysis of human brain using 13001-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 13001-1-AP (CRTAC1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse lung using 13001-1-AP

    IHC staining of mouse lung using 13001-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 13001-1-AP (CRTAC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse lung using 13001-1-AP

    IHC staining of mouse lung using 13001-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 13001-1-AP (CRTAC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 13001-1-AP

    IHC staining of mouse brain using 13001-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13001-1-AP (CRTAC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat lung using 13001-1-AP

    IHC staining of rat lung using 13001-1-AP

    Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 13001-1-AP (CRTAC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inhuman brain tissue
Positive IHC detected inmouse lung tissue, mouse brain tissue, rat lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

13001-1-AP targets CRTAC1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CRTAC1 fusion protein Ag3673 相同性解析による交差性が予測される生物種
Full Name cartilage acidic protein 1
Calculated molecular weight 661 aa, 71 kDa
Observed molecular weight 68-72 kDa
GenBank accession numberBC034245
Gene symbol CRTAC1
Gene ID (NCBI) 55118
RRIDAB_10638787
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CRTAC1 antibody 13001-1-APDownload protocol
IHC protocol for CRTAC1 antibody 13001-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Cell Rep

Integrative single-cell meta-analysis reveals disease-relevant vascular cell states and markers in human atherosclerosis

Authors - Jose Verdezoto Mosquera
View Article
humanWB

Am J Rhinol Allergy

Analysis of CRSsNP Proteome Using a Highly Multiplexed Approach in Nasal Mucus

Authors - Vanessa-Vivien Pesold
View Article