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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using C-Reactive Protein/CRP Recombinant monoclonal anti (86912-6-RR)

    WB analysis using 86912-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86912-6-RR (CRP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using CRP Recombinant monoclonal antibody (86912-6-RR)

    WB analysis using 86912-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86912-6-RR (CRP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using C-Reactive Protein/CRP Recombinant monoclonal anti (86912-6-RR)

    IHC staining of human liver using 86912-6-RR

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 86912-6-RR (C-Reactive Protein/CRP antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using C-Reactive Protein/CRP Recombinant monoclonal anti (86912-6-RR)

    IHC staining of human liver using 86912-6-RR

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 86912-6-RR (C-Reactive Protein/CRP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity of 86912-6-RR

    Affinity of 86912-6-RR

    Biolayer interferometry (BLI) kinetic assays of 86912-6-RR against Mouse C-Reactive Protein/CRP were performed. The affinity constant is below 1 pM.

C-Reactive Protein/CRP Recombinant monoclonal antibody

C-Reactive Protein/CRP Uni-rAb® Recombinant Antibody for WB, IHC, ELISA
Cat No. 86912-6-RR
Clone No.251971G6

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, ELISA

Synonyms

CRP, C reactive protein, C-Reactive Protein, C-reactive protein(1-205), PTX1

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman plasma, rat liver tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

86912-6-RR targets C-Reactive Protein/CRP in WB, IHC, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg2448

Product name: Recombinant Human C-Reactive Protein/CRP protein (rFc Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 19-224 aa of BC125135

Sequence: QTDMSRKAFVFPKESDTSYVSLKAPLTKPLKAFTVCLHFYTELSSTRGYSIFSYATKRQDNEILIFWSKDIGYSFTVGGSEILFEVPEVTVAPVHICTSWESASGIVEFWVDGKPRVRKSLKKGYTVGAEASIILGQEQDSFGGNFEGSQSLVGDIGNVNMWDFVLSPDEINTIYLGGPFSPNVLNWRALKYEVQGEVFTKPQLWP

相同性解析による交差性が予測される生物種
Full Name C-reactive protein, pentraxin-related
Calculated molecular weight 224 aa, 25 kDa
Observed molecular weight 25-28 kDa
GenBank accession numberBC125135
Gene Symbol CRP
Gene ID (NCBI) 1401
RRIDAB_3745201
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP02741
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

C-reactive protein (CRP) is an acute-phase serum protein synthesized by the liver in response to interleukin-6 (IL-6) during inflammation. The name of CRP derives from its ability to react with the C polysaccharide of Streptococcus pneumoniae. CRP is an annular, pentameric protein that belongs to the pentraxin family of proteins. CRP displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. It is used mainly as a marker of inflammation.

Protocols

Product Specific Protocols
IHC protocol for C-Reactive Protein/CRP antibody 86912-6-RRDownload protocol
WB protocol for C-Reactive Protein/CRP antibody 86912-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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