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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CROP Recombinant monoclonal antibody (86690-1-RR)

    WB analysis using 86690-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86690-1-RR (CROP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using CROP Recombinant monoclonal antibody (86690-1-RR)

    WB analysis using 86690-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86690-1-RR (CROP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CROP Recombinant monoclonal antibody (86690-1-RR)

    IF Staining of HepG2 using 86690-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CROP antibody (86690-1-RR, Clone: 251672C12 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 86690-1-RR

    Affinity and Kinetic Characterization of 86690-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86690-1-RR against Human CROP were performed. The affinity constant is 3.07 nM.

CROP Recombinant monoclonal antibody

CROP Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 86690-1-RR
Clone No.251672C12

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

cAMP regulatory element-associated protein 1, Cisplatin resistance-associated-overexpressed protein, CRE associated protein 1, CREAP 1, CREAP1

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, RT-4 cells, HepG2 cells, mouse brain tissue
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

86690-1-RR targets CROP in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag5961

Product name: Recombinant human CROP protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-79 aa of BC056409

Sequence: MISAAQLLDELMGRDRNLAPDEKRSNVRWDHESVCKYYLCGFCPAELFTNTRSDLDVFGRGDNISDVSKFLEDDKWMEE

相同性解析による交差性が予測される生物種
Full Name cisplatin resistance-associated overexpressed protein
Calculated molecular weight 51 kDa
Observed molecular weight 55-58 kDa
GenBank accession numberBC056409
Gene Symbol CROP
Gene ID (NCBI) 51747
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDO95232
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CROP, a putative SR protein, contain cystine/histidine motifs and leucine zipper-like repeats in the N-terminal half and consists mostly of charged and polar amino acids in the C-terminal half. CROP has a high expression in heart, brain, pancreas, thymus, and weak in lung, live and kidney. As the human homologue of yeast Luc7p, CROP is supported to be participated in 5'-splice site recognize and is essential for vegetative growth. This antibody raised against part of N-terminus of CROP of human origin, and can recognize a ~58kDa band of CROP.

Protocols

Product Specific Protocols
IF protocol for CROP antibody 86690-1-RRDownload protocol
WB protocol for CROP antibody 86690-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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