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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using CROP Polyclonal antibody (14504-1-AP)

    WB analysis of Jurkat using 14504-1-AP (same clone as 14504-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 14504-1-AP (CROP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14504-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using CROP Polyclonal antibody (14504-1-AP)

    WB analysis of HepG2 using 14504-1-AP (same clone as 14504-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 14504-1-AP (CROP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14504-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using CROP Polyclonal antibody (14504-1-AP)

    WB analysis of mouse brain using 14504-1-AP (same clone as 14504-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 14504-1-AP (CROP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14504-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using CROP Polyclonal antibody (14504-1-AP)

    WB analysis of HepG2 using 14504-1-AP (same clone as 14504-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 14504-1-AP (CROP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14504-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using CROP Polyclonal antibody (14504-1-AP)

    WB analysis of Jurkat using 14504-1-AP (same clone as 14504-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 14504-1-AP (CROP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14504-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HepG2 cells using CROP Polyclonal antibody (14504-1-AP)

    IP experiment of HepG2 using 14504-1-AP (same clone as 14504-1-PBS)

    IP result of anti-CROP (IP:14504-1-AP, 4ug; Detection:14504-1-AP 1:500) with HepG2 cells lysate 3600ug. This data was developed using the same antibody clone with 14504-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using CROP Polyclonal antibody (14504-1-AP)

    IHC staining of human ovary cancer using 14504-1-AP (same clone as 14504-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 14504-1-AP (CROP antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14504-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tissue using CROP Polyclonal antibody (14504-1-AP)

    IHC staining of human ovary using 14504-1-AP (same clone as 14504-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 14504-1-AP (CROP Antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 14504-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tissue using CROP Polyclonal antibody (14504-1-AP)

    IHC staining of human ovary using 14504-1-AP (same clone as 14504-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 14504-1-AP (CROP Antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 14504-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using CROP Polyclonal antibody (14504-1-AP)

    IHC staining of human pancreas cancer using 14504-1-AP (same clone as 14504-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14504-1-AP (CROP Antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 14504-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using CROP Polyclonal antibody (14504-1-AP)

    IHC staining of human pancreas cancer using 14504-1-AP (same clone as 14504-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14504-1-AP (CROP Antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 14504-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CROP Polyclonal antibody (14504-1-AP)

    IF Staining of HepG2 using 14504-1-AP (same clone as 14504-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CROP antibody (14504-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 14504-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

CROP Polyclonal antibody, PBS Only

CROP Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 14504-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

CREAP-1, CREAP1, CREAP 1, CRE associated protein 1, Cisplatin resistance-associated-overexpressed protein

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14504-1-PBS targets CROP in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag5961

Product name: Recombinant human CROP protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-79 aa of BC056409

Sequence: MISAAQLLDELMGRDRNLAPDEKRSNVRWDHESVCKYYLCGFCPAELFTNTRSDLDVFGRGDNISDVSKFLEDDKWMEE

相同性解析による交差性が予測される生物種
Full Name cisplatin resistance-associated overexpressed protein
Calculated molecular weight 51 kDa
Observed molecular weight 55-58 kDa
GenBank accession numberBC056409
Gene Symbol CROP
Gene ID (NCBI) 51747
RRIDAB_2229967
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO95232
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CROP, a putative SR protein, contain cystine/histidine motifs and leucine zipper-like repeats in the N-terminal half and consists mostly of charged and polar amino acids in the C-terminal half. CROP has a high expression in heart, brain, pancreas, thymus, and weak in lung, live and kidney. As the human homologue of yeast Luc7p, CROP is supported to be participated in 5'-splice site recognize and is essential for vegetative growth. This is a rabbit polyclonal antibody raised against part of N-terminus of CROP of human origin, and can recognize a ~58kDa band of CROP.

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