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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CRMP2 Polyclonal antibody (14686-1-AP)

    WB analysis using 14686-1-AP (same clone as 14686-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14686-1-AP (CRMP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14686-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CRMP2 Polyclonal antibody (14686-1-AP)

    WB analysis using 14686-1-AP (same clone as 14686-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14686-1-AP (CRMP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14686-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CRMP2 Polyclonal antibody (14686-1-AP)

    IHC staining of mouse brain using 14686-1-AP (same clone as 14686-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14686-1-AP (CRMP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14686-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CRMP2 Polyclonal antibody (14686-1-AP)

    IHC staining of mouse brain using 14686-1-AP (same clone as 14686-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14686-1-AP (CRMP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14686-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using CRMP2 Polyclonal antibody (14686-1-AP)

    IF Staining of SH-SY5Y using 14686-1-AP (same clone as 14686-1-PBS)

    Immunofluorescent analysis of SH-SY5Y cells using 14686-1-AP (CRMP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14686-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CRMP2 Polyclonal antibody (14686-1-AP)

    IF Staining of HepG2 using 14686-1-AP (same clone as 14686-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CRMP2 antibody (14686-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 14686-1-PBS in a different storage buffer formulation.

CRMP2 Polyclonal antibody, PBS Only

CRMP2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 14686-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14686-1-PBS targets CRMP2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag5985

Product name: Recombinant human DPYSL2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 223-572 aa of BC056408

Sequence: HVLSRPEEVEAEAVNRAITIANQTNCPLYITKVMSKSSAEVIAQARKKGTVVYGEPITASLGTDGSHYWSKNWAKAAAFVTSPPLSPDPTTPDFLNSLLSCGDLQVTGSAHCTFNTAQKAVGKDNFTLIPEGTNGTEERMSVIWDKAVVTGKMDENQFVAVTSTNAAKVFNLYPRKGRIAVGSDADLVIWDPDSVKTISAKTHNSSLEYNIFEGMECRGSPLVVISQGKIVLEDGTLHVTEGSGRYIPRKPFPDFVYKRIKARSRLAELRGVPRGLYDGPVCEVSVTPKTVTPASSAKTSPAKQQAPPVRNLHQSGFSLSGAQIDDNIPRRTTQRIVAPPGGRANITSLG

相同性解析による交差性が予測される生物種
Full Name dihydropyrimidinase-like 2
Calculated molecular weight 62 kDa
Observed molecular weight 62-66 kDa
GenBank accession numberBC056408
Gene Symbol CRMP2
Gene ID (NCBI) 1808
RRIDAB_10638925
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ16555
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Dihydropyrimidinase-like 2 (Dpysl2), a protein involved in neuronal differentiation and axonal guidance is found altered in various nervous system diseases (PMID:19524114). It is also named as CRMP2, ULIP2 and belongs to hydantoinase/dihydropyrimidinase subfamily. The phosphorylation of the particular portion carboxyl-terminal to the basic region of DPYSL2 may play an important role in regulating its activity, and may be involved in the formation of degenerating neurites in AD brain(PMID:10757975).

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