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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using CRK Monoclonal antibody (67035-1-Ig)

    WB analysis of HEK-293 using 67035-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67035-1-Ig (CRK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using CRK Monoclonal antibody (67035-1-Ig)

    WB analysis of Jurkat using 67035-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67035-1-Ig (CRK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HSC-T6 cells using CRK Monoclonal antibody (67035-1-Ig)

    WB analysis of HSC-T6 using 67035-1-Ig

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67035-1-Ig (CRK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using CRK Monoclonal antibody (67035-1-Ig)

    WB analysis of NIH/3T3 using 67035-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67035-1-Ig (CRK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of RAW 264.7 cells using CRK Monoclonal antibody (67035-1-Ig)

    WB analysis of RAW 264.7 using 67035-1-Ig

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 67035-1-Ig (CRK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using CRK Monoclonal antibody (67035-1-Ig)

    WB analysis of HeLa using 67035-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 67035-1-Ig (CRK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

CRK Monoclonal antibody

CRK Monoclonal Antibody for WB, ELISA
Cat No. 67035-1-Ig
Clone No.2B7E1

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

2B7E1, Adapter molecule crk, CRKII, p38, Proto oncogene c Crk

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Biotin
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, HSC-T6 cells, Jurkat cells, NIH/3T3 cells, RAW 264.7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67035-1-Ig targets CRK in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag28793

Product name: Recombinant human CRK protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 41-304 aa of BC008506

Sequence: STSPGDYVLSVSENSRVSHYIINSSGPRPPVPPSPAQPPPGVSPSRLRIGDQEFDSLPALLEFYKIHYLDTTTLIEPVSRSRQGSGVILRQEEAEYVRALFDFNGNDEEDLPFKKGDILRIRDKPEEQWWNAEDSEGKRGMIPVPYVEKYRPASASVSALIGGNQEGSHPQPLGGPEPGPYAQPSVNTPLPNLQNGPIYARVIQKRVPNAYDKTALALEVGELVKVTKINVSGQWEGECNGKRGHFPFTHVRLLDQQNPDEDFS

相同性解析による交差性が予測される生物種
Full Name v-crk sarcoma virus CT10 oncogene homolog (avian)
Calculated molecular weight 34 kDa
Observed molecular weight 34 kDa
GenBank accession numberBC008506
Gene Symbol CRK
Gene ID (NCBI) 1398
RRIDAB_2882350
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP46108
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CRK is a member of an adapter protein family that binds to several tyrosine-phosphorylated proteins. CRK has several SH2 and SH3 domains (src-homology domains) and is involved in several signaling pathways, recruiting cytoplasmic proteins in the vicinity of tyrosine kinase through SH2-phosphotyrosine interaction. The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation.

Protocols

Product Specific Protocols
WB protocol for CRK antibody 67035-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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