Validation Data Gallery

Filter: AllWBIPIHC

WB analysis using 14847-1-AP (same clone as 14847-1-PBS)
human saliva samples were subjected to SDS PAGE followed by western blot with 14847-1-AP (CRISP3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14847-1-PBS in a different storage buffer formulation.
WB analysis of mouse ovary using 14847-1-AP (same clone as 14847-1-PBS)
mouse ovary tissue were subjected to SDS PAGE followed by western blot with 14847-1-AP (CRISP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14847-1-PBS in a different storage buffer formulation.
WB analysis of mouse ovary using 14847-1-AP (same clone as 14847-1-PBS)
mouse ovary tissue were subjected to SDS PAGE followed by western blot with 14847-1-AP (CRISP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14847-1-PBS in a different storage buffer formulation.
WB analysis of mouse colon using 14847-1-AP (same clone as 14847-1-PBS)
mouse colon tissue were subjected to SDS PAGE followed by western blot with 14847-1-AP (CRISP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14847-1-PBS in a different storage buffer formulation.
WB analysis of mouse colon using 14847-1-AP (same clone as 14847-1-PBS)
mouse colon tissue were subjected to SDS PAGE followed by western blot with 14847-1-AP (CRISP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14847-1-PBS in a different storage buffer formulation.
WB analysis of mouse thymus using 14847-1-AP (same clone as 14847-1-PBS)
mouse thymus tissue were subjected to SDS PAGE followed by western blot with 14847-1-AP (CRISP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14847-1-PBS in a different storage buffer formulation.
WB analysis of human placenta using 14847-1-AP (same clone as 14847-1-PBS)
human placenta tissue were subjected to SDS PAGE followed by western blot with 14847-1-AP (CRISP3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14847-1-PBS in a different storage buffer formulation.
WB analysis of Jurkat using 14847-1-AP (same clone as 14847-1-PBS)
Jurkat cells were subjected to SDS PAGE followed by western blot with 14847-1-AP (CRISP3 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14847-1-PBS in a different storage buffer formulation.
IP experiment of human placenta using 14847-1-AP (same clone as 14847-1-PBS)
IP result of anti-CRISP3 (IP:14847-1-AP, 4ug; Detection:14847-1-AP 1:300) with human placenta tissue lysate 1520ug. This data was developed using the same antibody clone with 14847-1-PBS in a different storage buffer formulation.
IHC staining of human prostate cancer using 14847-1-AP (same clone as 14847-1-PBS)
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14847-1-AP (CRISP3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14847-1-PBS in a different storage buffer formulation.
IHC staining of human prostate cancer using 14847-1-AP (same clone as 14847-1-PBS)
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14847-1-AP (CRISP3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14847-1-PBS in a different storage buffer formulation.
IHC staining of human pancreas using 14847-1-AP (same clone as 14847-1-PBS)
Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 14847-1-AP (CRISP3 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14847-1-PBS in a different storage buffer formulation.
IHC staining of human pancreas using 14847-1-AP (same clone as 14847-1-PBS)
Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 14847-1-AP (CRISP3 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14847-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

CRISP3 Polyclonal antibody, PBS Only

CRISP3 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 14847-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14847-1-PBS targets CRISP3 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide 相同性解析による交差性が予測される生物種
Full Name	cysteine-rich secretory protein 3
Calculated molecular weight	28 kDa
Observed molecular weight	29 kDa
GenBank accession number	NM_006061
Gene Symbol	CRISP3
Gene ID (NCBI)	10321
RRID	AB_1607510
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P54108
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

Background Information

CRISP3, also known as SGP28, is a glycoprotein that belongs to a family of cysteine-rich secretory proteins (CRISPs). CRISP3 was originally discovered in human neutrophils, but transcripts are widely distributed in exocrine glands (salivary glands, pancreas, and prostate) and also found at lower levels in epididymis, ovary, thymus, and colon. CRISP3 is a potential biomarker for prostate cancer. This antibody can recognize both CRISP2 and CRISP3.

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 14847-1-AP (same clone as 14847-1-PBS)

WB analysis of mouse ovary using 14847-1-AP (same clone as 14847-1-PBS)

WB analysis of mouse ovary using 14847-1-AP (same clone as 14847-1-PBS)

WB analysis of mouse colon using 14847-1-AP (same clone as 14847-1-PBS)

WB analysis of mouse colon using 14847-1-AP (same clone as 14847-1-PBS)

WB analysis of mouse thymus using 14847-1-AP (same clone as 14847-1-PBS)

WB analysis of human placenta using 14847-1-AP (same clone as 14847-1-PBS)

WB analysis of Jurkat using 14847-1-AP (same clone as 14847-1-PBS)

IP experiment of human placenta using 14847-1-AP (same clone as 14847-1-PBS)

IHC staining of human prostate cancer using 14847-1-AP (same clone as 14847-1-PBS)