Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse testis tissue, human testis tissue, rat testis tissue |
| Positive IP detected in | mouse testis tissue |
| Positive IHC detected in | human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 8 publications below |
| IF | See 4 publications below |
| IP | See 1 publications below |
Product Information
19066-1-AP targets CRISP2 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag13574 Product name: Recombinant human CRISP2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 27-243 aa of BC022011 Sequence: TALLTTQLQVQREIVNKHNELRKAVSPPASNMLKMEWSREVTTNAQRWANKCTLQHSDPEDRKTSTRCGENLYMSSDPTSWSSAIQSWYDEILDFVYGVGPKSPNAVVGHYTQLVWYSTYQVGCGIAYCPNQDSLKYYYVCQYCPAGNNMNRKNTPYQQGTPCAGCPDDCDKGLCTNSCQYQDLLSNCDSLKNTAGCEHELLKEKCKATCLCENKIY 相同性解析による交差性が予測される生物種 |
| Full Name | cysteine-rich secretory protein 2 |
| Calculated molecular weight | 27 kDa |
| Observed molecular weight | 27 kDa |
| GenBank accession number | BC022011 |
| Gene Symbol | CRISP2 |
| Gene ID (NCBI) | 7180 |
| RRID | AB_10667008 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P16562 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for CRISP2 antibody 19066-1-AP | Download protocol |
| IP protocol for CRISP2 antibody 19066-1-AP | Download protocol |
| WB protocol for CRISP2 antibody 19066-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun PNLDC1 is essential for piRNA 3' end trimming and transposon silencing during spermatogenesis in mice. | ||
J Anim Sci Biotechnol Single-cell transcriptomics reveals the cellular dynamics of hexafluoropropylene oxide dimer acid in exerting mouse male reproductive toxicity | ||
FASEB J Functional redundancy and compensation: Deletion of multiple murine Crisp genes reveals their essential role for male fertility. | ||
Mol Hum Reprod Fertilization defects in sperm from Cysteine-rich secretory protein 2 (Crisp2) knockout mice: implications for fertility disorders.
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