Validation Data Gallery

Filter: AllWBIPIHC

WB analysis using 19066-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 19066-1-AP (CRISP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of human testis using 19066-1-AP
human testis tissue were subjected to SDS PAGE followed by western blot with 19066-1-AP (CRISP2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of human testis using 19066-1-AP
human testis tissue were subjected to SDS PAGE followed by western blot with 19066-1-AP (CRISP2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IP experiment of mouse testis using 19066-1-AP
IP result of anti-CRISP2 (IP:19066-1-AP, 4ug; Detection:19066-1-AP 1:300) with mouse testis tissue lysate 4000ug.
IHC staining of human testis using 19066-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 19066-1-AP (CRISP2 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human testis using 19066-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 19066-1-AP (CRISP2 antibody) at dilution of 1:50 (under 40x lens).

Featured Product
KD/KO Validated

CRISP2 Polyclonal antibody

CRISP2 Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 19066-1-AP

Publications(10)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, IP, ELISA

Synonyms

Cancer/testis antigen 36, CRISP 2, CRISP2, CT36, GAPDL5, Testis specific protein TPX 1, TPX1, TSP1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information
Publications (10)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse testis tissue, human testis tissue, rat testis tissue
Positive IP detected in	mouse testis tissue
Positive IHC detected in	human testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 8 publications below
IF	See 4 publications below
IP	See 1 publications below

Product Information

19066-1-AP targets CRISP2 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, pig
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag13574 Product name: Recombinant human CRISP2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 27-243 aa of BC022011 Sequence: TALLTTQLQVQREIVNKHNELRKAVSPPASNMLKMEWSREVTTNAQRWANKCTLQHSDPEDRKTSTRCGENLYMSSDPTSWSSAIQSWYDEILDFVYGVGPKSPNAVVGHYTQLVWYSTYQVGCGIAYCPNQDSLKYYYVCQYCPAGNNMNRKNTPYQQGTPCAGCPDDCDKGLCTNSCQYQDLLSNCDSLKNTAGCEHELLKEKCKATCLCENKIY 相同性解析による交差性が予測される生物種
Full Name	cysteine-rich secretory protein 2
Calculated molecular weight	27 kDa
Observed molecular weight	27 kDa
GenBank accession number	BC022011
Gene Symbol	CRISP2
Gene ID (NCBI)	7180
RRID	AB_10667008
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P16562
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IHC protocol for CRISP2 antibody 19066-1-AP	Download protocol
IP protocol for CRISP2 antibody 19066-1-AP	Download protocol
WB protocol for CRISP2 antibody 19066-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IF	Nat Commun PNLDC1 is essential for piRNA 3' end trimming and transposon silencing during spermatogenesis in mice. Authors - Deqiang Ding View Article
mouse	WB	Nucleic Acids Res DAZAP1 regulates the splicing of Crem, Crisp2 and Pot1a transcripts. Authors - Chen Hsiang-Ying HY View Article
mouse	WB	FASEB J Functional redundancy and compensation: Deletion of multiple murine Crisp genes reveals their essential role for male fertility. Authors - L Curci View Article
human	WB	Biol Reprod The Expression of Cysteine-Rich Secretory Protein 2 (CRISP2) and Its Specific Regulator miR-27b in the Spermatozoa of Patients with Asthenozoospermia. Authors - Jun-Hao Zhou View Article
mouse	WB	Mol Hum Reprod Fertilization defects in sperm from Cysteine-rich secretory protein 2 (Crisp2) knockout mice: implications for fertility disorders. Authors - N G Brukman KO Validated View Article
pig	IF,WB	Biol Reprod Characterization of different oligomeric forms of CRISP2 in the perinuclear theca versus the fibrous tail structures of boar spermatozoa. Authors - M Zhang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 19066-1-AP

WB analysis of human testis using 19066-1-AP

WB analysis of human testis using 19066-1-AP

IP experiment of mouse testis using 19066-1-AP

IHC staining of human testis using 19066-1-AP

IHC staining of human testis using 19066-1-AP

CRISP2 Polyclonal antibody

CRISP2 Polyclonal Antibody for WB, IP, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

PNLDC1 is essential for piRNA 3' end trimming and transposon silencing during spermatogenesis in mice.

DAZAP1 regulates the splicing of Crem, Crisp2 and Pot1a transcripts.

Functional redundancy and compensation: Deletion of multiple murine Crisp genes reveals their essential role for male fertility.

The Expression of Cysteine-Rich Secretory Protein 2 (CRISP2) and Its Specific Regulator miR-27b in the Spermatozoa of Patients with Asthenozoospermia.

Fertilization defects in sperm from Cysteine-rich secretory protein 2 (Crisp2) knockout mice: implications for fertility disorders.

Characterization of different oligomeric forms of CRISP2 in the perinuclear theca versus the fibrous tail structures of boar spermatozoa.