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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CRIP1 Recombinant antibody (83340-3-RR)

    WB analysis using 83340-3-RR (same clone as 83340-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83340-3-RR (CRIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83340-3-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP00382-3

    Sandwich ELISA standard curve of MP00382-3

    Sandwich ELISA standard curve of MP00382-3, Human CRIP1 Recombinant Matched Antibody Pair - PBS only. 83340-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag7588. 83340-3-PBS was HRP conjugated as the detection antibody. Range: 46.88-3000 pg/mL.

  • Sample test of MP00382-3

    Sample test of MP00382-3

    The mean CRIP1 concentration was determined to be 3,364.0 pg/mL in PC-3 cell extract based on a 3.0 mg/mL extract load and 3,015.0 pg/mL in HeLa cell extract based on a 3.0 mg/mL extract load.

  • Immunohistochemistry (IHC) staining of human colon tissue using CRIP1 Recombinant antibody (83340-3-RR)

    IHC staining of human colon using 83340-3-RR (same clone as 83340-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 83340-3-RR (CRIP1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83340-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83340-3-PBS

    Affinity and Kinetic Characterization of 83340-3-PBS

    Biolayer interferometry (BLl) kinetic assay of 83340-3-PBS against Human CRIP1 was performed. The affinity constant is 0.693 nM.

  • Affinity and Kinetic Characterization of 83340-3-RR

    Affinity and Kinetic Characterization of 83340-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83340-3-RR against Human CRIP1 were performed. The affinity constant is 0.784 nM.

CRIP1 Recombinant monoclonal antibody, PBS Only (Detector)

CRIP1 Uni-rAb® Recombinant Antibody for WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 83340-3-PBS
Clone No.240176G2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

240176G2, CRHP, CRIP, CRIP 1, CRP 1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83340-3-PBS targets CRIP1 as part of a matched antibody pair:

MP00382-3: 83340-4-PBS capture and 83340-3-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag7588

Product name: Recombinant human CRIP1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-77 aa of BC002738

Sequence: MPKCPKCNKEVYFAERVTSLGKDWHRPCLKCEKCGKTLTSGGHAEHEGKPYCNHPCYAAMFGPKGFGRGGAESHTFK

相同性解析による交差性が予測される生物種
Full Name cysteine-rich protein 1 (intestinal)
Calculated molecular weight 9 kDa
Observed molecular weight 8-9 kDa
GenBank accession numberBC002738
Gene Symbol CRIP1
Gene ID (NCBI) 1396
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP50238
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CRIP1 also known as CRIP, CRP1, belongs to the LIM/double zinc finger protein family. CRIP1 has a unique double zinc finger motif, which is mainly expressed in the intestine, and may be involved in intestinal zinc transport (PMID: 31312368, 1946385). CRIP1 is overexpressed in immune cells of the epithelium and may play an important role in gut immunity(PMID: 27836662).

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