Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, SH-SY5Y cells, Y79 cells, HT-29 cells, rat brain tissue, mouse brain tissue, mouse liver tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
87114-1-RR targets CRHR1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | corticotropin releasing hormone receptor 1 |
| Calculated molecular weight | 48 kDa |
| Observed molecular weight | 68 kDa |
| GenBank accession number | NM_004382 |
| Gene Symbol | CRHR1 |
| Gene ID (NCBI) | 1394 |
| RRID | AB_3745349 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P34998 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Corticotropin-releasing hormone (CRH, also known as Corticotropin-releasing factor, CRF) is a 41 amino acid peptide hormone and a key regulator of the stress response. CRH and the related urocortin peptides (UCN, UCN2 and UCN3) mediate their actions through two mammalian receptor subtypes, CRHR1 and CRHR2 (PMID: 23863939). CRHR1 (also known as CRFR1) exhibits high affinity towards CRH and UCN but low affinity towards UCN2 and no affinity towards UCN3 (PMID: 22684850). The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. Widely distributed throughout the brain, CRHR1 critically controls behavioral adaptation to stress and is causally linked to emotional disorders (PMID: 21885734). Alternative splicing results in multiple transcript variants encoding distinct isoforms of CRHR1. This antibody raised against a synthesized peptide corresponding to amino acid residues 148-165 of isoform CRF-R1 can only recognize isoform CRF-R1.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CRHR1 antibody 87114-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |