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Validation Data Gallery

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  • Western Blot (WB) analysis of NIH/3T3 cells using CREST Monoclonal antibody (60314-1-Ig)

    WB analysis of NIH/3T3 using 60314-1-Ig (same clone as 60314-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 60314-1-Ig (CREST antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60314-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of RAW 264.7 cells using CREST Monoclonal antibody (60314-1-Ig)

    WB analysis of RAW 264.7 using 60314-1-Ig (same clone as 60314-1-PBS)

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 60314-1-Ig (CREST antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60314-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using CREST Monoclonal antibody (60314-1-Ig)

    WB analysis of MCF-7 using 60314-1-Ig (same clone as 60314-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 60314-1-Ig (CREST antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60314-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of COLO 320 cells using CREST Monoclonal antibody (60314-1-Ig)

    WB analysis of COLO 320 using 60314-1-Ig (same clone as 60314-1-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 60314-1-Ig (CREST antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60314-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HSC-T6 cells using CREST Monoclonal antibody (60314-1-Ig)

    WB analysis of HSC-T6 using 60314-1-Ig (same clone as 60314-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 60314-1-Ig (CREST antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60314-1-PBS in a different storage buffer formulation.

  • Cytometric bead array sample test of MP51462-1

    Cytometric bead array sample test of MP51462-1

    Cytometric bead array sample test of MP51462-1, CREST Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60314-1-PBS. Detection antibody: 60314-2-PBS.

  • Cytometric bead array standard curve of MP51462-1

    Cytometric bead array standard curve of MP51462-1

    Cytometric bead array standard curve of MP51462-1, CREST Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60314-1-PBS. Detection antibody: 60314-2-PBS. Standard:Ag3119. Range: 0.781-100 ng/mL.

CREST Monoclonal antibody, PBS Only (Capture)

CREST Monoclonal Antibody for WB, Cytometric bead array, Indirect ELISA
Cat No. 60314-1-PBS
Clone No.3D5D10

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, Cytometric bead array, Indirect ELISA

Synonyms

SS18L1, 3D5D10, Calcium-responsive transactivator, KIAA0693, LP2261

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60314-1-PBS targets CREST as part of a matched antibody pair:

MP51462-1: 60314-1-PBS capture and 60314-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag3119

Product name: Recombinant human CREST protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 47-396 aa of BC034494

Sequence: CTQYQQILHRNLVYLATIADSNQNMQSLLPAPPTQNMNLGPGALTQSGSSQGLHSQGSLSDAISTGLPPSSLLQGQIGNGPSHVSMQQTAPNTLPTTSMSISGPGYSHAGPASQGVPMQGQGTIGNYVSRTNINMQSNPVSMIQQQAATSHYSSAQGGSQHYQGQSSIAMMGQGSQGSSMMGQRPMAPYRPSQQGSSQQYLGQEEYYGEQYSHSQGAAEPMGQQYYPDGHGDYAYQQSSYTEQSYDRSFEESTQHYYEGGNSQYSQQQAGYQQGAAQQQTYSQQQYPSQQSYPGQQQGYGSAQGAPSQYPGYQQGQGQQYGSYRAPQTAPSAQQQRPYGYEQGQYGNYQQ

相同性解析による交差性が予測される生物種
Full Name synovial sarcoma translocation gene on chromosome 18-like 1
Calculated molecular weight 396 aa, 43 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC034494
Gene Symbol CREST
Gene ID (NCBI) 26039
RRIDAB_2881425
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDO75177
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

SS18-like 1(SS18L1) is a transcriptional activator that is required for calcium-dependent dendritic growth and branching in cortical neurons. It's also a nuclear protein interacts with CREB-binding protein and expressed in the developing brain. It helps regulate neuronal morphogenesis in calcuim-dependent manner. The N-terminal domain of SS18L1 is required for suppressing transactivation in the basal state, while the C-terminal domain is required for calcium-induced transactivation. It's part of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. This complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP. The calculated molecular weight of CREST is about 43 kDa, but the modified of CREST protein is 55 kDa (PMID: 25888396).

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