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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CREST Polyclonal antibody (12439-1-AP)

    WB analysis using 12439-1-AP (same clone as 12439-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12439-1-AP (CREST antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12439-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CREST Polyclonal antibody (12439-1-AP)

    WB analysis using 12439-1-AP (same clone as 12439-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12439-1-AP (CREST antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12439-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using CREST Polyclonal antibody (12439-1-AP)

    WB analysis of HeLa using 12439-1-AP (same clone as 12439-1-PBS)

    WB result of CREST antibody (12439-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CREST transfected HeLa cells. This data was developed using the same antibody clone with 12439-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of COLO 320 cells using CREST Polyclonal antibody (12439-1-AP)

    WB analysis of COLO 320 using 12439-1-AP (same clone as 12439-1-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 12439-1-AP (CREST antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12439-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human heart tissue using CREST Polyclonal antibody (12439-1-AP)

    WB analysis of human heart using 12439-1-AP (same clone as 12439-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 12439-1-AP (CREST antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12439-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using CREST Polyclonal antibody (12439-1-AP)

    IP experiment of HeLa using 12439-1-AP (same clone as 12439-1-PBS)

    IP result of anti-CREST (IP:12439-1-AP, 4ug; Detection:12439-1-AP 1:300) with HeLa cells lysate 3000 ug. This data was developed using the same antibody clone with 12439-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using CREST Polyclonal antibody (12439-1-AP)

    IP experiment of mouse brain using 12439-1-AP (same clone as 12439-1-PBS)

    IP result of anti-CREST (IP:12439-1-AP, 3ug; Detection:12439-1-AP 1:1000) with mouse brain tissue lysate 6000ug. This data was developed using the same antibody clone with 12439-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CREST Polyclonal antibody (12439-1-AP)

    IHC staining of human breast cancer using 12439-1-AP (same clone as 12439-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 12439-1-AP (CREST antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12439-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using CREST Polyclonal antibody (12439-1-AP)

    IHC staining of human brain using 12439-1-AP (same clone as 12439-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 12439-1-AP (CREST antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12439-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using CREST Polyclonal antibody (12439-1-AP)

    IHC staining of human brain using 12439-1-AP (same clone as 12439-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 12439-1-AP (CREST antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12439-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CREST Polyclonal antibody (12439-1-AP)

    IHC staining of human breast cancer using 12439-1-AP (same clone as 12439-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 12439-1-AP (CREST antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12439-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CREST Polyclonal antibody (12439-1-AP)

    IHC staining of human breast cancer using 12439-1-AP (same clone as 12439-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 12439-1-AP (CREST antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12439-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CREST Polyclonal antibody (12439-1-AP)

    IHC staining of human breast cancer using 12439-1-AP (same clone as 12439-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 12439-1-AP (CREST antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12439-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CREST Polyclonal antibody (12439-1-AP)

    IHC staining of human breast cancer using 12439-1-AP (same clone as 12439-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 12439-1-AP (CREST antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12439-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CREST Polyclonal antibody (12439-1-AP)

    IHC staining of human breast cancer using 12439-1-AP (same clone as 12439-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 12439-1-AP (CREST antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 12439-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CREST Polyclonal antibody (12439-1-AP)

    IHC staining of human breast cancer using 12439-1-AP (same clone as 12439-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 12439-1-AP (CREST antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 12439-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CREST Polyclonal antibody (12439-1-AP)

    IHC staining of human breast cancer using 12439-1-AP (same clone as 12439-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 12439-1-AP (CREST antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 12439-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CREST Polyclonal antibody (12439-1-AP)

    IHC staining of human breast cancer using 12439-1-AP (same clone as 12439-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 12439-1-AP (CREST antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 12439-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using CREST Polyclonal antibody (12439-1-AP)

    IF Staining of mouse testis using 12439-1-AP (same clone as 12439-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using 12439-1-AP (CREST antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12439-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using CREST Polyclonal antibody (12439-1-AP)

    IF Staining of mouse testis using 12439-1-AP (same clone as 12439-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using 12439-1-AP (CREST antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12439-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CREST Polyclonal antibody (12439-1-AP)

    IF Staining of HeLa using 12439-1-AP (same clone as 12439-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CREST antibody (12439-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), (CL594-Phalloidin, red). This data was developed using the same antibody clone with 12439-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

CREST Polyclonal antibody, PBS Only

CREST Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA
Cat No. 12439-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, IP, ELISA

Synonyms

SS18L1, Calcium-responsive transactivator, KIAA0693, LP2261, SS18 like protein 1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12439-1-PBS targets CREST in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3119

Product name: Recombinant human CREST protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 47-396 aa of BC034494

Sequence: CTQYQQILHRNLVYLATIADSNQNMQSLLPAPPTQNMNLGPGALTQSGSSQGLHSQGSLSDAISTGLPPSSLLQGQIGNGPSHVSMQQTAPNTLPTTSMSISGPGYSHAGPASQGVPMQGQGTIGNYVSRTNINMQSNPVSMIQQQAATSHYSSAQGGSQHYQGQSSIAMMGQGSQGSSMMGQRPMAPYRPSQQGSSQQYLGQEEYYGEQYSHSQGAAEPMGQQYYPDGHGDYAYQQSSYTEQSYDRSFEESTQHYYEGGNSQYSQQQAGYQQGAAQQQTYSQQQYPSQQSYPGQQQGYGSAQGAPSQYPGYQQGQGQQYGSYRAPQTAPSAQQQRPYGYEQGQYGNYQQ

相同性解析による交差性が予測される生物種
Full Name synovial sarcoma translocation gene on chromosome 18-like 1
Calculated molecular weight 396 aa, 43 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC034494
Gene Symbol CREST
Gene ID (NCBI) 26039
RRIDAB_2302517
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO75177
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CREST, also named as SS18-like 1(SS18L1) is a transcriptional activator that is required for calcium-dependent dendritic growth and branching in cortical neurons. It's also a nuclear protein interacts with CREB-binding protein and expressed in the developing brain. It helps regulate neuronal morphogenesis in calcuim-dependent manner. The N-terminal domain of SS18L1 is required for suppressing transactivation in the basal state, while the C-terminal domain is required for calcium-induced transactivation. It's part of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. This complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP. The calculated molecular weight of CREST is about 43 kDa, but the modified of CREST protein is 55 kDa (PMID: 25888396). CREST exists some isoforms with calulated MV 43, 40, 33 and 29 kDa.

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