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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CRELD1 Polyclonal antibody

CRELD1 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 29903-1-AP

Synonyms

AVSD2, CIRRIN, CRELD1, DKFZp566D213

Validation Data Gallery

Applications:
  • WB analysis of HepG2 using 29903-1-AP

    WB analysis of HepG2 using 29903-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 29903-1-AP (CRELD1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of rat lung using 29903-1-AP

    WB analysis of rat lung using 29903-1-AP

    rat lung tissue were subjected to SDS PAGE followed by western blot with 29903-1-AP (CRELD1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse lung using 29903-1-AP

    WB analysis of mouse lung using 29903-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 29903-1-AP (CRELD1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse heart using 29903-1-AP

    IHC staining of mouse heart using 29903-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 29903-1-AP (CRELD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of U2OS using 29903-1-AP

    IF Staining of U2OS using 29903-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CRELD1 antibody (29903-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHepG2 cells, mouse lung tissue, rat lung tissue
Positive IHC detected inmouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inU2OS cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

29903-1-AP targets CRELD1 in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CRELD1 fusion protein Ag31593 相同性解析による交差性が予測される生物種
Full Name cysteine-rich with EGF-like domains 1
Calculated molecular weight45KD
Observed molecular weight45 kDa, 70-75 kDa
GenBank accession numberNM_001031717
Gene symbol CRELD1
Gene ID (NCBI) 78987
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CRELD1 (Cysteine-rich with EGF-like domain protein 1) belongs to matricellular proteins family. The encoded protein has a cysteine-rich with epidermal growth factor-like domain (PubMed: 12137942). Mutations in CRELD1 are the cause of atrioventricular septal defect (PubMed: 24697899). Alternate splicing results in multiple transcript variants. CRELD1 can be detected as 70-75 kDa in mouse lung (PMID: 24697899).

Protocols

Product Specific Protocols
WB protocol for CRELD1 antibody 29903-1-APDownload protocol
IHC protocol for CRELD1 antibody 29903-1-APDownload protocol
IF protocol for CRELD1 antibody 29903-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols