Validation Data Gallery
Tested Applications
| Positive WB detected in | HepG2 cells, mouse lung tissue, rat lung tissue |
| Positive IHC detected in | mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
29903-1-AP targets CRELD1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.
| Tested Reactivity | Human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag31593 Product name: Recombinant human CRELD1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 218-362 aa of NM_001031717 Sequence: CARCSGPEESNCLQCKKGWALHHLKCVDIDECGTEGANCGADQFCVNTEGSYECRDCAKACLGCMGAGPGRCKKCSPGYQQVGSKCLDVDECETEVCPGENKQCENTEGGYRCICAEGYKQMEGICVKEQIPESAGFFSEMTEDE 相同性解析による交差性が予測される生物種 |
| Full Name | cysteine-rich with EGF-like domains 1 |
| Calculated molecular weight | 45KD |
| Observed molecular weight | 45 kDa, 70-75 kDa |
| GenBank accession number | NM_001031717 |
| Gene Symbol | CRELD1 |
| Gene ID (NCBI) | 78987 |
| RRID | AB_2923619 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96HD1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CRELD1 (Cysteine-rich with EGF-like domain protein 1) belongs to matricellular proteins family. The encoded protein has a cysteine-rich with epidermal growth factor-like domain (PubMed: 12137942). Mutations in CRELD1 are the cause of atrioventricular septal defect (PubMed: 24697899). Alternate splicing results in multiple transcript variants. CRELD1 can be detected as 70-75 kDa in mouse lung (PMID: 24697899).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CRELD1 antibody 29903-1-AP | Download protocol |
| IHC protocol for CRELD1 antibody 29903-1-AP | Download protocol |
| WB protocol for CRELD1 antibody 29903-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |