Various lysates were subjected to Tris-Acetate gel system followed by western blot with 22277-1-AP (CREBBP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22277-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22277-1-AP (CREBBP antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22277-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22277-1-AP (CREBBP antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22277-1-PBS in a different storage buffer formulation.

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CREBBP antibody (22277-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 22277-1-PBS in a different storage buffer formulation.

1x10^6 HeLa cells were intracellularly stained with 0.25 ug CREBBP Polyclonal antibody (22277-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 22277-1-PBS in a different storage buffer formulation.