Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, human brain tissue, MCF-7 cells, fetal human brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 7 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
14196-1-AP targets CREB5 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, chicken |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag5399 Product name: Recombinant human CREB5 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-369 aa of BC059400 Sequence: MFCTSGGNSASVMSMRPVPGSLSSLLHLHNRQRQPMPASMPGTLPNPTMPGSSAVLMPMERQMSVNSSIMGMQGPNLSNPCASPQVQPMHSEAKMRLKAALTHHPAAMSNGNMNTMGHMMEMMGSRQDQTPHHHMHSHPHQHQTLPPHHPYPHQHQHPAHHPHPQPHHQQNHPHHHSHSHLHAHPAHHQTSPHPPLHTGNQAQVSPATQQMQPTQTIQPPQPTGGRRRRVVDEDPDERRRKFLERNRAAATRCRQKRKVWVMSLEKKAEELTQTNMQLQNEVSMLKNEVAQLKQLLLTHKDCPITAMQKESQGYLSPESSPPASPVPACSQQQVIQHNTITTSSSVSEVVGSSTLSQLTTHRTDLNPIL 相同性解析による交差性が予測される生物種 |
| Full Name | cAMP responsive element binding protein 5 |
| Calculated molecular weight | 57 kDa |
| Observed molecular weight | 57~68 kDa |
| GenBank accession number | BC059400 |
| Gene Symbol | CREB5 |
| Gene ID (NCBI) | 9586 |
| RRID | AB_2083832 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q02930 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
cyclic AMP responsive element binding protein 5 (CREB5), also known as CRE-BPA, a member of the cAMP response element binding protein family, is a transcription factor specifically expressed in superficial zone articular chondrocytes and is required for TGF-β and EGFR signaling. Knockdown of CREB5 decreased the c-jun and c-fos expression levels in HC-a cells (PMID: 38549717). Increased CREB5 expression is positively correlated with tumor cell invasion and a poor prognosis for cancer patients with epithelial ovarian cancer and hepatocellular carcinoma (PMID: 38418476).This protein specifically binds to CRE as a homodimer or a heterodimer with c-Jun or CRE-BP1, and functions as a CRE-dependent trans-activator. Western analysis detected bands at 57~68 kDa possibly due to PTM (PMID: 33712729, 37816820).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CREB5 antibody 14196-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Int J Hyperthermia Hyperthermia promotes exosome secretion by regulating Rab7b while increasing drug sensitivity in adriamycin-resistant breast cancer. | ||
J Ovarian Res Role of BMI1 in epithelial ovarian cancer: investigated via the CRISPR/Cas9 system and RNA sequencing. | ||
Cancer Med Long noncoding RNA SNHG4 promotes the malignant progression of hepatocellular carcinoma through the miR-211-5p/CREB5 axis | ||
Int J Med Sci Kisspeptins inhibit ectopic endometrial cell invasion and angiogenesis by suppressing PI3K/AKT signaling pathway via CREB5 in endometriosis.
| ||
PLoS One AT-hook DNA-binding motif-containing protein one knockdown downregulates EWS-FLI1 transcriptional activity in Ewing's sarcoma cells | ||
J Anim Sci Biotechnol Unique Sertoli cell adaptations support enhanced spermatogenesis in chickens. |