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Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using CREB3L1,OASIS Polyclonal antibody (11235-2-AP)

    WB analysis of A549 using 11235-2-AP (same clone as 11235-2-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 11235-2-AP (CREB3L1,OASIS antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This batch of A549 cells lysate predominantly expressed the maturer form likely due to cell stage. This data was developed using the same antibody clone with 11235-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of COLO 320 cells using CREB3L1,OASIS Polyclonal antibody (11235-2-AP)

    WB analysis of COLO 320 using 11235-2-AP (same clone as 11235-2-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 11235-2-AP (CREB3L1,OASIS antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11235-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using CREB3L1,OASIS Polyclonal antibody (11235-2-AP)

    WB analysis of A549 using 11235-2-AP (same clone as 11235-2-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 11235-2-AP (CREB3L1,OASIS antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This batch of A549 cells lysate predominantly expressed the precursor form likely due to cell stage. This data was developed using the same antibody clone with 11235-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using CREB3L1,OASIS Polyclonal antibody (11235-2-AP)

    IHC staining of human prostate cancer using 11235-2-AP (same clone as 11235-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11235-2-AP (CREB3L1,OASIS antibody) at dilution of 1:50 (under 25x lens). This data was developed using the same antibody clone with 11235-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using CREB3L1,OASIS Polyclonal antibody (11235-2-AP)

    IF Staining of A549 using 11235-2-AP (same clone as 11235-2-PBS)

    Immunofluorescent analysis of (10% Formaldehyde) fixed A549 cells using 11235-2-AP (CREB3L1,OASIS antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11235-2-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

CREB3L1,OASIS Polyclonal antibody, PBS Only

CREB3L1,OASIS Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 11235-2-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11235-2-PBS targets CREB3L1,OASIS in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag1757

Product name: Recombinant human CREB3L1,OASIS protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 170-519 aa of BC015781

Sequence: PHQAPGEMTQLPVIKAEPLEVNQFLKVTPEDLVQMPPTPPSSHGSDSDGSQSPRSLPPSSPVRPMARSSTAISTSPLLTAPHKLQGTSGPLLLTEEEKRTLIAEGYPIPTKLPLTKAEEKALKRVRRKIKNKISAQESRRKKKEYVECLEKKVETFTSENNELWKKVETLENANRTLLQQLQKLQTLVTNKISRPYKMAATQTGTCLMVAALCFVLVLGSLVPCLPEFSSGSQTVKEDPLAADGVYTASQMPSRSLLFYDDGAGLWEDGRSTLLPMEPPDGWEINPGGPAEQRPRDHLQHDHLDSTHETTKYLSEAWPKDGGNGTSPDFSHSKEWFHDRDLGPNTTIKLS

相同性解析による交差性が予測される生物種
Full Name cAMP responsive element binding protein 3-like 1
Calculated molecular weight 57 kDa
Observed molecular weight 55-58 kDa and 84 kDa
GenBank accession numberBC015781
Gene Symbol CREB3L1/OASIS
Gene ID (NCBI) 90993
RRIDAB_2086042
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96BA8
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Cyclic AMP-responsive element-binding protein 3-like protein 1(CREB3L1) belongs to a family of transcription factors that are synthesized as membrane-bound precursors in the endoplasmic reticulum (ER) and then transported to the Golgi where they are activated through regulated intramembrane proteolysis (RIP). Also it participates in ER-stress response in astrocytes in the central nervous system. It may has a role in gliosis, and involves in activation of transcription through box-B element but not CRE. CREB3L1 acts as a transcription repressor in proliferation of virus-infected cell. The full-length precursor form (approximately 84 kDa) of CREB3L1 and the matured form of CREB3L1 is 58 kDa. (PMID: 20668028)

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