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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CREB3L1,OASIS Monoclonal antibody

CREB3L1,OASIS Monoclonal Antibody for WB, FC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, FC, ELISA

Conjugate

Unconjugated

CloneNo.

2B5B4

Cat no : 67617-1-Ig

Synonyms

CREB3L1, CREB3L1,OASIS, OASIS

Validation Data Gallery

Applications:
  • WB analysis using 67617-1-Ig

    WB analysis using 67617-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67617-1-Ig (CREB3L1,OASIS antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • WB analysis using 67617-1-Ig

    WB analysis using 67617-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67617-1-Ig (CREB3L1,OASIS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of HSC-T6 using 67617-1-Ig

    WB analysis of HSC-T6 using 67617-1-Ig

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67617-1-Ig (CREB3L1,OASIS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of NIH/3T3 using 67617-1-Ig

    WB analysis of NIH/3T3 using 67617-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67617-1-Ig (CREB3L1,OASIS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis using 67617-1-Ig

    WB analysis using 67617-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67617-1-Ig (CREB3L1,OASIS antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

  • FC experiment of HepG2 using 67617-1-Ig

    FC experiment of HepG2 using 67617-1-Ig

    1X10^6 HepG2 cells were intracellularly stained with 0.5 ug Anti-Human CREB3L1,OASIS (67617-1-Ig, Clone:2B5B4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inHepG2 cells, HeLa cells, HSC-T6 cells, NIH/3T3 cells, A375 cells, A549 cells, 4T1 cells
Positive FC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy CREB3L1 Ready-To-Use IHC Kit. CREB3L1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Flow Cytometry (FC)FC : 0.50 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

67617-1-Ig targets CREB3L1,OASIS in WB, FC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CREB3L1,OASIS fusion protein Ag28042 相同性解析による交差性が予測される生物種
Full Name cAMP responsive element binding protein 3-like 1
Calculated molecular weight 57 kDa
Observed molecular weight 58 kDa
GenBank accession numberBC015781
Gene symbol CREB3L1
Gene ID (NCBI) 90993
RRIDAB_2882820
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cyclic AMP-responsive element-binding protein 3-like protein 1(CREB3L1) belongs to a family of transcription factors that are synthesized as membrane-bound precursors in the endoplasmic reticulum (ER) and then transported to the Golgi where they are activated through regulated intramembrane proteolysis (RIP). Also it participates in ER-stress response in astrocytes in the central nervous system. It may has a role in gliosis, and involves in activation of transcription through box-B element but not CRE. CREB3L1 acts as a transcription repressor in proliferation of virus-infected cell. The full-length precursor form (approximately 84 kDa) of CREB3L1 and the matured form of CREB3L1 is 58 kDa. (PMID: 20668028)

Protocols

Product Specific Protocols
WB protocol for CREB3L1,OASIS antibody 67617-1-IgDownload protocol
FC protocol for CREB3L1,OASIS antibody 67617-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols