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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using CREB3 Polyclonal antibody (11275-1-AP)

    WB analysis of Jurkat using 11275-1-AP (same clone as 11275-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 11275-1-AP (CREB3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11275-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using CREB3 Polyclonal antibody (11275-1-AP)

    WB analysis of HeLa using 11275-1-AP (same clone as 11275-1-PBS)

    WB result of CREB3 antibody (11275-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-CREB3 transfected HeLa cells. This data was developed using the same antibody clone with 11275-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using CREB3 Polyclonal antibody (11275-1-AP)

    IHC staining of human prostate cancer using 11275-1-AP (same clone as 11275-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11275-1-AP (CREB3 antibody) at dilution of 1:50 (under 25x lens). This data was developed using the same antibody clone with 11275-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using CREB3 Polyclonal antibody (11275-1-AP)

    IF Staining of U2OS using 11275-1-AP (same clone as 11275-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CREB3 antibody (11275-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11275-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

CREB3 Polyclonal antibody, PBS Only

CREB3 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 11275-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11275-1-PBS targets CREB3 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag1823

Product name: Recombinant human CREB3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-371 aa of BC009402

Sequence: MELELDAGDQDLLAFLLEESGDLGTAPDEAVRAPLDWALPLSEVPSDWEVDDLLCSLLSPPASLNILSSSNPCLVHHDHTYSLPRETVSMDLESESCRKEGTQMTPQHMEELAEQEIARLVLTDEEKSLLEKEGLILPETLPLTKTEEQILKRVRRKIRNKRSAQESRRKKKVYVGGLESRVLKYTAQNMELQNKVQLLEEQNLSLLDQLRKLQAMVIEISNKTSSSSTCILVLLVSFCLLLVPAIYSSDTRGSLPAEHGVLSRQLRALPSEDPYQLELPALQSEVPKDSTHQWLDGSDCVLQAPGNTSCLLHYMPQAPSAEPPLEWPFPDLFSEPLCRGPILPLQANLTRKGGWLPTGSPSVILQDRYSG

相同性解析による交差性が予測される生物種
Full Name cAMP responsive element binding protein 3
Calculated molecular weight 44 kDa
Observed molecular weight 40-64 kDa
GenBank accession numberBC009402
Gene Symbol CREB3
Gene ID (NCBI) 10488
RRIDAB_10640533
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO43889
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CREB3, also named as LZIP and Luman, belongs to the bZIP family and ATF subfamily. It is a transcription factors activated upon intramembrane proteolysis (RIP), binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AG][AG]-3'), a sequence present in many viral and cellular promoters. CREB3 binds to and requires HCFC1 as a coactivator. The activity and expression are suppressed when the HCFC1-CREB3 complex binds with CREBZF. Participates in LKN-1/CCL15-induced chemotaxis signaling. CREB3 is N-glycosylated and released by proteolysis. So the MW of CREB3 emirate 40kd to 64kd (PMID: 12138176).

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