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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CRBN Polyclonal antibody (28494-1-AP)

    WB analysis using 28494-1-AP (same clone as 28494-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28494-1-AP (CRBN antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28494-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CRBN Polyclonal antibody (28494-1-AP)

    WB analysis using 28494-1-AP (same clone as 28494-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28494-1-AP (CRBN antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28494-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CRBN Polyclonal antibody (28494-1-AP)

    WB analysis using 28494-1-AP (same clone as 28494-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28494-1-AP (CRBN antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28494-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using CRBN Polyclonal antibody (28494-1-AP)

    WB analysis of mouse liver using 28494-1-AP (same clone as 28494-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 28494-1-AP (CRBN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28494-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CRBN Polyclonal antibody (28494-1-AP)

    IHC staining of human liver cancer using 28494-1-AP (same clone as 28494-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28494-1-AP (CRBN antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28494-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CRBN Polyclonal antibody (28494-1-AP)

    IHC staining of human liver cancer using 28494-1-AP (same clone as 28494-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28494-1-AP (CRBN antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28494-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CRBN Polyclonal antibody (28494-1-AP)

    IHC staining of mouse brain using 28494-1-AP (same clone as 28494-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28494-1-AP (CRBN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28494-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CRBN Polyclonal antibody (28494-1-AP)

    IHC staining of mouse brain using 28494-1-AP (same clone as 28494-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28494-1-AP (CRBN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28494-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of COLO 320 cells using CRBN Polyclonal antibody (28494-1-AP)

    IF Staining of COLO 320 using 28494-1-AP (same clone as 28494-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed COLO 320 cells using CRBN antibody (28494-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 28494-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

CRBN Polyclonal antibody, PBS Only

CRBN Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 28494-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Protein cereblon, cereblon, AD-006

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

28494-1-PBS targets CRBN in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CRBN fusion protein Ag29353 相同性解析による交差性が予測される生物種
Full Name cereblon
Calculated molecular weight 442 aa, 51 kDa
Observed molecular weight 51 kDa
GenBank accession numberBC017419
Gene Symbol CRBN
Gene ID (NCBI) 51185
RRIDAB_2881155
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96SW2
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CRBN plays an important role in assembly and surface expression of functional BK(Ca) channels. The mutation of human cereblon gene (CRBN) is related with mild mental retardation, and causes ARNSID (nonsyndromal intellectual disability) by distubing the developmental regulation of BK(Ca) in brain regions that are critical for memory and learning. CRBN is also identified as important thalidomide-binding protein for limb outgrowth in zebrafish and chicks. It can be detected a band above 51 kDa (maybe 55 kDa) in HT22 cells(PMID: 21232561).