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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse brain tissue using CRB2 Polyclonal antibody (28015-1-AP)

    IHC staining of mouse brain using 28015-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28015-1-AP (CRB2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse eye tissue using CRB2 Polyclonal antibody (28015-1-AP)

    IF Staining of mouse eye using 28015-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using CRB2 antibody (28015-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

CRB2 Polyclonal antibody

CRB2 Polyclonal Antibody for IHC, IF-P, ELISA
Cat No. 28015-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF-P, ELISA

Synonyms

Crumbs homolog 2, crumbs homolog 2 (Drosophila), Crumbs like protein 2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse eye tissue

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28015-1-AP targets CRB2 in IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CRB2 fusion protein Ag27826 相同性解析による交差性が予測される生物種
Full Name crumbs homolog 2 (Drosophila)
Calculated molecular weight 134 aa
GenBank accession numberNM_173689
Gene Symbol CRB2
Gene ID (NCBI) 286204
RRIDAB_3669636
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5IJ48
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CRB2 (Crumbs homolog-2) has a large extracellular domain with epidermal growth-factor-like and laminin-A globular domains, a single transmembrane domain, and an intracellular C-terminal domain of 37 amino acids (PMID: 31438467). CRB2 is a candidate modifying gene of human CRB1-related retinal dystrophy (PMID: 24565864).

Protocols

Product Specific Protocols
IHC protocol for CRB2 antibody 28015-1-APDownload protocol
IF protocol for CRB2 antibody 28015-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols