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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CRACR2A Monoclonal antibody (66787-1-Ig)

    WB analysis using 66787-1-Ig (same clone as 66787-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66787-1-Ig (CRACR2A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66787-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using CRACR2A Monoclonal antibody (66787-1-Ig)

    WB analysis of Jurkat using 66787-1-Ig (same clone as 66787-1-PBS)

    WB result of CRACR2A antibody (66787-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CRACR2A transfected Jurkat cells. This data was developed using the same antibody clone with 66787-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using CRACR2A Monoclonal antibody (66787-1-Ig)

    IHC staining of human colon using 66787-1-Ig (same clone as 66787-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66787-1-Ig (CRACR2A antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66787-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using CRACR2A Monoclonal antibody (66787-1-Ig)

    IF Staining of HEK-293 using 66787-1-Ig (same clone as 66787-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using 66787-1-Ig (CRACR2A antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66787-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

CRACR2A Monoclonal antibody, PBS Only

CRACR2A Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66787-1-PBS
Clone No.4C1F5

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

EFCAB4B, EF-hand calcium-binding domain-containing protein 4B, CRAC channel regulator 2A, Calcium release-activated channel regulator 2A, Calcium release-activated calcium channel regulator 2A

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66787-1-PBS targets CRACR2A in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag7525

Product name: Recombinant human EFCAB4B protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-395 aa of BC004524

Sequence: MAAPDGRVVSRPQRLGQGSGQGPKGSGACLHPLDSLEQKETQEQTSGQLVMLRKAQEFFQTCDAEGKGFIARKDMQRLHKELPLSLEELEDVFDALDADGNGYLTPQEFTTGFSHFFFSQNNPSQEDAGEQVAQRHEEKVYLSRGDEDLGDMGEDEEAQFRMLMDRLGAQKVLEDESDVKQLWLQLKKEEPHLLSNFEDFLTRIISQLQEAHEEKNELECALKRKIAAYDEEIQHLYEEMEQQIKSEKEQFLLKDTERFQARSQELEQKLLCKEQELEQLTQKQKRLEGQCTALHHDKHETKAENTKLKLTNQELARELERTSWELQDAQQQLESLQQEACKLHQEKEMEVYRVTESLQREKAGLLKQLDFLRCVGGHWPVLRAPPRSLGSEGPV

相同性解析による交差性が予測される生物種
Full Name EF-hand calcium binding domain 4B
Calculated molecular weight 46 kDa
Observed molecular weight 80-90 kDa
GenBank accession numberBC004524
Gene Symbol CRACR2A
Gene ID (NCBI) 84766
RRIDAB_2882132
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDQ9BSW2
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CRACR2A (also called CRAC regulator 2A, EFCAB4B) is a cytoplasmic Ca2+ sensor predominantly expressed in lymphocytes. It has two isoforms: a long isoform of CRACR2A-a round 80-90 kDa and a short isoform of CRACR2A-c around 46 kDa. CRACR2A-c is involved in the regulation of Ca2+-NFAT signaling. CRACR2A-a exists in a GTP- and Golgi membrane-bound form in resting T cells, while it is present in subsynaptic vesicles that translocated from the Golgi region to the immunological synapse after ligation of TCRs. This antibody recognizes both of CRACR2A-c and CRACR2A-a.

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