Validation Data Gallery

Filter: AllWBIPIHCIF-PIF/ICCFC (Intra)

WB analysis of HeLa using 10225-1-AP
WB result of CRABP2 antibody (10225-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-CRABP2 transfected HeLa cells.
WB analysis using 10225-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10225-1-AP (CRABP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 10225-1-AP
IP result of anti-CRABP2 (IP:10225-1-AP, 3ug; Detection:10225-1-AP 1:300) with HeLa cells lysate 4650ug.
IHC staining of mouse embryo using 10225-1-AP
Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 10225-1-AP (CRABP2 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse embryo using 10225-1-AP
Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 10225-1-AP (CRABP2 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of human skin cancer using 10225-1-AP
Immunofluorescent analysis of (4% PFA) fixed human skin cancer tissue using CRABP2 antibody (10225-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of MCF-7 using 10225-1-AP
Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CRABP2 antibody (10225-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).
FC experiment of MCF-7 using 10225-1-AP
1X10^6 MCF-7 cells were intracellularly stained with 0.2 ug Anti-Human CRABP2 (10225-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

Featured Product
KD/KO Validated

CRABP2 Polyclonal antibody

CRABP2 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Cat No. 10225-1-AP

Publications(51)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ELISA

Synonyms

Cellular retinoic acid binding protein II, Cellular retinoic acid-binding protein 2, Cellular retinoic acid-binding protein II, CRABP II, CRABP-II

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite®594

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (51)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HEK-293 cells, HeLa cells, HT-29 cells, MCF-7 cells, mouse embryo tissue
Positive IP detected in	HeLa cells
Positive IHC detected in	mouse embryo tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	human skin cancer tissue
Positive IF/ICC detected in	MCF-7 cells
Positive FC (Intra) detected in	MCF-7 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:150-1:600
Immunofluorescence (IF)-P	IF-P : 1:200-1:800
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 7 publications below
WB	See 33 publications below
IHC	See 15 publications below
IF	See 15 publications below
IP	See 4 publications below
CoIP	See 2 publications below

Product Information

10225-1-AP targets CRABP2 in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag0309 Product name: Recombinant human CRABP2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-138 aa of BC001109 Sequence: MPNFSGNWKIIRSENFEELLKVLGVNVMLRKIAVAAASKPAVEIKQEGDTFYIKTSTTVRTTEINFKVGEEFEEQTVDGRPCKSLVKWESENKMVCEQKLLKGEGPKTSWTRELTNDGELILTMTADDVVCTRVYVRE 相同性解析による交差性が予測される生物種
Full Name	cellular retinoic acid binding protein 2
Calculated molecular weight	16 kDa
Observed molecular weight	16 kDa
GenBank accession number	BC001109
Gene Symbol	CRABP2
Gene ID (NCBI)	1382
RRID	AB_2085455
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P29373
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Cellular retinoic acid binding protein 2 (CRABP2, synonyms: RBP6, CRABP-II). A number of specific carrier proteins for members of the vitamin A family have been discovered. Cellular retinoic acid binding proteins (CRABP) are low molecular weight proteins whose precise function remains unknown. CRABP2 is important in retinoic acid-mediated regulation of human skin growth and differentiation. It has been postulated that the CRABP2 gene is transcriptionally regulated by a newly synthesized regulatory protein.

Protocols

Product Specific Protocols
IF protocol for CRABP2 antibody 10225-1-AP	Download protocol
IHC protocol for CRABP2 antibody 10225-1-AP	Download protocol
IP protocol for CRABP2 antibody 10225-1-AP	Download protocol
WB protocol for CRABP2 antibody 10225-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IF	Science A single progenitor population switches behavior to maintain and repair esophageal epithelium. Authors - David P Doupé View Article
human	WB	Mol Cell TRIP12 promotes small-molecule-induced degradation through K29/K48-branched ubiquitin chains. Authors - Ai Kaiho-Soma View Article
human	WB,IF	Neuron Divergent Hox Coding and Evasion of Retinoid Signaling Specifies Motor Neurons Innervating Digit Muscles. Authors - Alana I Mendelsohn View Article
mouse	IF	Nat Commun The developing mouse coronal suture at single-cell resolution. Authors - D'Juan T Farmer View Article
mouse	IF	Dev Cell Single-Cell Transcriptomic Analyses of the Developing Meninges Reveal Meningeal Fibroblast Diversity and Function. Authors - John DeSisto View Article
mouse	WB	Proc Natl Acad Sci U S A UTX (KDM6A) promotes differentiation noncatalytically in somatic self-renewing epithelia Authors - Gina N Pacella View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HeLa using 10225-1-AP

WB analysis using 10225-1-AP

IP experiment of HeLa using 10225-1-AP

IHC staining of mouse embryo using 10225-1-AP

IHC staining of mouse embryo using 10225-1-AP

IF Staining of human skin cancer using 10225-1-AP

IF Staining of MCF-7 using 10225-1-AP

FC experiment of MCF-7 using 10225-1-AP

CRABP2 Polyclonal antibody

CRABP2 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

A single progenitor population switches behavior to maintain and repair esophageal epithelium.

TRIP12 promotes small-molecule-induced degradation through K29/K48-branched ubiquitin chains.

Divergent Hox Coding and Evasion of Retinoid Signaling Specifies Motor Neurons Innervating Digit Muscles.

The developing mouse coronal suture at single-cell resolution.

Single-Cell Transcriptomic Analyses of the Developing Meninges Reveal Meningeal Fibroblast Diversity and Function.

UTX (KDM6A) promotes differentiation noncatalytically in somatic self-renewing epithelia