CPT2 Polyclonal antibody
CPT2 Polyclonal Antibody for WB, IP, IF, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IP, IF, IHC, ELISA
Conjugate
Unconjugated
Cat no : 26555-1-AP
Synonyms
Validation Data Gallery
Applications:
Tested Applications
| Positive WB detected in | HEK-293T cells, mouse heart tissue, T-47D cells, MCF-7 cells, LNCaP cells, mouse liver tissue, rat liver tissue |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | C2C12 cells |
Planning an IHC experiment? We recommend our IHCeasy CPT2 Ready-To-Use IHC Kit. CPT2 primary antibody included.
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 24 publications below |
| IHC | See 3 publications below |
| IF | See 1 publications below |
| IP | See 4 publications below |
Product Information
26555-1-AP targets CPT2 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, rat, mouse, bovine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CPT2 fusion protein Ag24897 相同性解析による交差性が予測される生物種 |
| Full Name | carnitine palmitoyltransferase 2 |
| Calculated molecular weight | 74 kDa |
| Observed molecular weight | 65-70 kDa |
| GenBank accession number | BC002445 |
| Gene symbol | CPT2 |
| Gene ID (NCBI) | 1376 |
| RRID | AB_2880551 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CPT2 antibody 26555-1-AP | Download protocol |
| IHC protocol for CPT2 antibody 26555-1-AP | Download protocol |
| IF protocol for CPT2 antibody 26555-1-AP | Download protocol |
| IP protocol for CPT2 antibody 26555-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Metab Targeting Erbin-mitochondria axis in platelets/megakaryocytes promotes B cell-mediated antitumor immunity | ||
Cell Stem Cell A small-molecule cocktail promotes mammalian cardiomyocyte proliferation and heart regeneration. | ||
Nat Commun Hyodeoxycholic acid ameliorates nonalcoholic fatty liver disease by inhibiting RAN-mediated PPARα nucleus-cytoplasm shuttling | ||
Theranostics Mitochondrial STAT3 exacerbates LPS-induced sepsis by driving CPT1a-mediated fatty acid oxidation. | ||
Elife Remodeling of whole-body lipid metabolism and a diabetic-like phenotype caused by loss of CDK1 and hepatocyte division. | ||
J Nanobiotechnology Regulating immune memory and reversing tumor thermotolerance through a step-by-step starving-photothermal therapy |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Mitchell (Verified Customer) (09-26-2019) | Worked great for western blot in proximal intestine and liver.
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