Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, Jurkat cells, HEK-293T cells, PC-3 cells, NIH/3T3 cells, C6 cells |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
88362-1-RR targets CPSF6 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag7852 Product name: Recombinant human CPSF6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-351 aa of BC000714 Sequence: MADGVDHIDIYADVGEEFNQEAEYGGHDQIDLYDDVISPSANNGDAPEDRDYMDTLPPTVGDDVGKGAAPNVVYTYTGKRIALYIGNLTWWTTDEDLTEAVHSLGVNDILEIKFFENRANGQSKGFALVGVGSEASSKKLMDLLPKRELHGQNPVVTPCNKQFLSQFEMQSRKTTQSGQMSGEGKAGPPGGSSRAAFPQGGRGRGRFPGAVPGGDRFPGPAGPGGPPPPFPGNLIKHLVKGTRPLFLETRIPWHMGHSIEEIPIFGLKAGQTPPRPPLGPPGPPGPPGPPPPGQVLPPPLAGPPNRGDRPPPPVLFPGQPFGQPPLGPLPPGPPPPVPGYGPPPGPPPPQQ 相同性解析による交差性が予測される生物種 |
| Full Name | cleavage and polyadenylation specific factor 6, 68kDa |
| Calculated molecular weight | 59 kDa |
| Observed molecular weight | 55-68 kDa |
| GenBank accession number | BC000714 |
| Gene Symbol | CPSF6 |
| Gene ID (NCBI) | 11052 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q16630 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The binding of Cleavage factor Im (CFIM), also known as CPSF6, to the pre-mRNA is one of the earliest steps in the assembly of the cleavage and polyadenylation machinery and facilitates the recruitment of other processing factors. CFIM is required for the first step in pre-mRNA 3′-end processing and can be reconstituted in vitro from its heterologously expressed 25- and 68-kDa subunits. It involved in RNA binding, protein-protein interactions, and subcellular localization [PMID:15169763]. In addition, it is a pre-mRNA processing protein that dynamically shuttles between the nucleus and the cytoplasm and contains a C-terminal nuclear-targeting arginine/serine-rich (RS-) domain of the type bound by TNPO3[PMID:15169763,19864460].
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CPSF6 antibody 88362-1-RR | Download protocol |
| WB protocol for CPSF6 antibody 88362-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |