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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CPSF3 Polyclonal antibody (11609-1-AP)

    WB analysis using 11609-1-AP (same clone as 11609-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11609-1-AP (CPSF3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11609-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using CPSF3 Polyclonal antibody (11609-1-AP)

    WB analysis of human brain using 11609-1-AP (same clone as 11609-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 11609-1-AP (CPSF3 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11609-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using CPSF3 Polyclonal antibody (11609-1-AP)

    WB analysis of HepG2 using 11609-1-AP (same clone as 11609-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 11609-1-AP (CPSF3 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11609-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using CPSF3 Polyclonal antibody (11609-1-AP)

    WB analysis of HEK-293 using 11609-1-AP (same clone as 11609-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 11609-1-AP (CPSF3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11609-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using CPSF3 Polyclonal antibody (11609-1-AP)

    IP experiment of HEK-293 using 11609-1-AP (same clone as 11609-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 11609-1-AP (CPSF3 antibody) at dilution of 1:700. This data was developed using the same antibody clone with 11609-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using CPSF3 Polyclonal antibody (11609-1-AP)

    IHC staining of mouse kidney using 11609-1-AP (same clone as 11609-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11609-1-AP (CPSF3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11609-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using CPSF3 Polyclonal antibody (11609-1-AP)

    IHC staining of mouse cerebellum using 11609-1-AP (same clone as 11609-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 11609-1-AP (CPSF3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11609-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using CPSF3 Polyclonal antibody (11609-1-AP)

    IHC staining of human thyroid cancer using 11609-1-AP (same clone as 11609-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 11609-1-AP (CPSF3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11609-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using CPSF3 Polyclonal antibody (11609-1-AP)

    IHC staining of human placenta using 11609-1-AP (same clone as 11609-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 11609-1-AP (CPSF3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11609-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

CPSF3 Polyclonal antibody, PBS Only

CPSF3 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 11609-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11609-1-PBS targets CPSF3 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag2173

Product name: Recombinant human CPSF3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 337-684 aa of BC020211

Sequence: SRELFESWCTDKRNGVIIAGYCVEGTLAKHIMSEPEEITTMSGQKLPLKMSVDYISFSAHTDYQQTSEFIRALKPPHVILVHGEQNEMARLKAALIREYEDNDEVHIEVHNPRNTEAVTLNFRGEKLAKVMGFLADKKPEQGQRVSGILVKRNFNYHILSPCDLSNYTDLAMSTVKQTQAIPYTGPFNLLCYQLQKLTGDVEELEIQEKPALKVFKNITVIQEPGMVVLEWLANPSNDMYADTVTTVILEVQSNPKIRKGAVQKVSKKLEMHVYSKRLEIMLQDIFGEDCVSVKDDSILSVTVDGKTANLNLETRTVECEEGSEDDESLREMVELAAQRLYEALTPVH

相同性解析による交差性が予測される生物種
Full Name cleavage and polyadenylation specific factor 3, 73kDa
Calculated molecular weight 684 aa, 77 kDa
Observed molecular weight 77 kDa
GenBank accession numberBC020211
Gene Symbol CPSF3
Gene ID (NCBI) 51692
RRIDAB_2292188
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UKF6
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CPSF3 (cleavage and polyadenylation specificity factor 3) is involved in human disease and is the putative target of benzoxaboroles in trypanosomatid parasites and Cryptosporidium infection. CPSF3 has also been associated with cancer malignancy in that CPSF3 induces cell cycle arrest in hepatocellular carcinoma and promotes lung adenocarcinoma cell proliferation and metastasis (PMID: 38718887). CPSF3 has been implicated in various cancers, including lung cancer, colorectal, prostate, and triple-negative breast cancers (PMID: 37627085).

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