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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using CPSF1 Polyclonal antibody (11031-1-AP)

    WB analysis of Jurkat using 11031-1-AP (same clone as 11031-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 11031-1-AP (CPSF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11031-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CPSF1 Polyclonal antibody (11031-1-AP)

    WB analysis using 11031-1-AP (same clone as 11031-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11031-1-AP (CPSF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11031-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using CPSF1 Polyclonal antibody (11031-1-AP)

    WB analysis of HeLa using 11031-1-AP (same clone as 11031-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 11031-1-AP (CPSF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11031-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using CPSF1 Polyclonal antibody (11031-1-AP)

    WB analysis of human brain using 11031-1-AP (same clone as 11031-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 11031-1-AP (CPSF1 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11031-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using CPSF1 Polyclonal antibody (11031-1-AP)

    IHC staining of mouse testis using 11031-1-AP (same clone as 11031-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11031-1-AP (CPSF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11031-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using CPSF1 Polyclonal antibody (11031-1-AP)

    IHC staining of mouse testis using 11031-1-AP (same clone as 11031-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11031-1-AP (CPSF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11031-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CPSF1 Polyclonal antibody (11031-1-AP)

    IHC staining of mouse brain using 11031-1-AP (same clone as 11031-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11031-1-AP (CPSF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11031-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CPSF1 Polyclonal antibody (11031-1-AP)

    IHC staining of mouse brain using 11031-1-AP (same clone as 11031-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11031-1-AP (CPSF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11031-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CPSF1 Polyclonal antibody (11031-1-AP)

    IHC staining of mouse brain using 11031-1-AP (same clone as 11031-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11031-1-AP (CPSF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11031-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

CPSF1 Polyclonal antibody, PBS Only

CPSF1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 11031-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11031-1-PBS targets CPSF1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag1489

Product name: Recombinant human CPSF1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1039-1443 aa of BC017232

Sequence: STNTPCARIPRMTGEEKEFETIERDERYIHPQQEAFSIQLISPVSWEAIPNARIELQEWEHVTCMKTVSLRSEETVSGLKGYVAAGTCLMQGEEVTCRGRILIMDVIEVVPEPGQPLTKNKFKVLYEKEQKGPVTALCHCNGHLVSAIGQKIFLWSLRASELTGMAFIDTQLYIHQMISVKNFILAADVMKSISLLRYQEESKTLSLVSRDAKPLEVYSVDFMVDNAQLGFLVSDRDRNLMVYMYLPEAKESFGGMRLLRRADFHVGAHVNTFWRTPCRGATEGLSKKSVVWENKHITWFATLDGGIGLLLPMQEKTYRRLLMLQNALTTMLPHHAGLNPRAFRMLHVDRRTLQNAVRNVLDGELLNRYLYLSTMERSELAKKIGTTPDIILDDLLETDRVTAHF

相同性解析による交差性が予測される生物種
Full Name cleavage and polyadenylation specific factor 1, 160kDa
Calculated molecular weight 160 kDa
Observed molecular weight 160 kDa
GenBank accession numberBC017232
Gene Symbol CPSF1
Gene ID (NCBI) 29894
RRIDAB_2245276
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ10570
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Cleavage and polyadenylation specificity factor (CPSF) is a multisubunit complex that plays a central role in 3-prime processing of pre-mRNAs. CPSF recognizes the AAUAAA signal in the pre-mRNA and interacts with other proteins to facilitate both RNA cleavage and poly(A) synthesis.

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