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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using CPS1 Polyclonal antibody (24513-1-AP)

    WB analysis of HeLa using 24513-1-AP

    WB result of CPS1 antibody (24513-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CPS1 transfected HeLa cells.

  • Western Blot (WB) analysis of HepG2 cells using CPS1 Polyclonal antibody (24513-1-AP)

    WB analysis of HepG2 using 24513-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 24513-1-AP (CPS1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using CPS1 Polyclonal antibody (24513-1-AP)

    WB analysis using 24513-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24513-1-AP (CPS1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat liver tissue using CPS1 Polyclonal antibody (24513-1-AP)

    WB analysis of rat liver using 24513-1-AP

    rat liver tissue were subjected to SDS PAGE followed by western blot with 24513-1-AP (CPS1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using CPS1 Polyclonal antibody (24513-1-AP)

    WB analysis of mouse liver using 24513-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 24513-1-AP (CPS1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CPS1 Polyclonal antibody (24513-1-AP)

    IHC staining of human liver cancer using 24513-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24513-1-AP (CPS1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO Validated

CPS1 Polyclonal antibody

CPS1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 24513-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Carbamoyl-phosphate synthase [ammonia], mitochondrial, Carbamoyl-phosphate synthetase I, CPSase I, EC:6.3.4.16

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, HeLa cells, mouse liver tissue, rat liver tissue, L02 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24513-1-AP targets CPS1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag21605

Product name: Recombinant human CPS1 protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 1361-1500 aa of BC140943

Sequence: GILIGIQQSFRPRFLGVAEQLHNEGFKLFATEATSDWLNANNVPATPVAWPSQEGQNPSLSSIRKLIRDGSIDLVINLPNNNTKFVHDNYVIRRTAVDSGIPLLTNFQVTKLFAEAVQKSRKVDSKSLFHYRQYSAGKAA

相同性解析による交差性が予測される生物種
Full Name carbamoyl-phosphate synthetase 1, mitochondrial
Calculated molecular weight 1500 aa, 165 kDa
Observed molecular weight140-165 kDa
GenBank accession numberBC140943
Gene Symbol CPS1
Gene ID (NCBI) 1373
RRIDAB_2879583
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP31327
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Carbamoyl phosphate synthetase 1 (CPS1) is the first rate-limiting enzyme in the urea cycle, converting ammonium into carbamoyl phosphate, and plays an intricate role in arginine metabolism and pyrimidine metabolism (PMID: 28376202). CPS1 expression is liver specific and suppressed in HCC cells and tumor tissues at the transcriptional level(PMID: 21281797). CPS1 gene comprises 38 exons that encode a polypeptide of 1500 amino acids and has a molecular weight of 165 kDa.

Protocols

Product Specific Protocols
IHC protocol for CPS1 antibody 24513-1-APDownload protocol
WB protocol for CPS1 antibody 24513-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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