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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CPOX Polyclonal antibody (16724-1-AP)

    WB analysis using 16724-1-AP (same clone as 16724-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16724-1-AP (CPOX antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16724-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using CPOX Polyclonal antibody (16724-1-AP)

    WB analysis of K-562 using 16724-1-AP (same clone as 16724-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 16724-1-AP (CPOX antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16724-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat liver tissue using CPOX Polyclonal antibody (16724-1-AP)

    WB analysis of rat liver using 16724-1-AP (same clone as 16724-1-PBS)

    rat liver tissue were subjected to SDS PAGE followed by western blot with 16724-1-AP (CPOX antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16724-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using CPOX Polyclonal antibody (16724-1-AP)

    WB analysis of HepG2 using 16724-1-AP (same clone as 16724-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 16724-1-AP (CPOX antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16724-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using CPOX Polyclonal antibody (16724-1-AP)

    WB analysis of K-562 using 16724-1-AP (same clone as 16724-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 16724-1-AP (CPOX antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16724-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using CPOX Polyclonal antibody (16724-1-AP)

    WB analysis of mouse liver using 16724-1-AP (same clone as 16724-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 16724-1-AP (CPOX antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16724-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using CPOX Polyclonal antibody (16724-1-AP)

    WB analysis of HepG2 using 16724-1-AP (same clone as 16724-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 16724-1-AP (CPOX antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16724-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human liver tissue using CPOX Polyclonal antibody (16724-1-AP)

    WB analysis of human liver using 16724-1-AP (same clone as 16724-1-PBS)

    human liver tissue were subjected to SDS PAGE followed by western blot with 16724-1-AP (CPOX antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16724-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HepG2 cells using CPOX Polyclonal antibody (16724-1-AP)

    IP experiment of HepG2 using 16724-1-AP (same clone as 16724-1-PBS)

    IP result of anti-CPOX (IP:16724-1-AP, 4ug; Detection:16724-1-AP 1:1000) with HepG2 cells lysate 1800ug. This data was developed using the same antibody clone with 16724-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using CPOX Polyclonal antibody (16724-1-AP)

    IHC staining of human liver using 16724-1-AP (same clone as 16724-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 16724-1-AP (CPOX antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 16724-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using CPOX Polyclonal antibody (16724-1-AP)

    IHC staining of human liver using 16724-1-AP (same clone as 16724-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 16724-1-AP (CPOX antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 16724-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using CPOX Polyclonal antibody (16724-1-AP)

    IHC staining of human testis using 16724-1-AP (same clone as 16724-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 16724-1-AP (CPOX antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 16724-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using CPOX Polyclonal antibody (16724-1-AP)

    IHC staining of human testis using 16724-1-AP (same clone as 16724-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 16724-1-AP (CPOX antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 16724-1-PBS in a different storage buffer formulation.

CPOX Polyclonal antibody, PBS Only

CPOX Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 16724-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16724-1-PBS targets CPOX in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag10164

Product name: Recombinant human CPOX protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 95-454 aa of BC017210

Sequence: HVQRAEMLPKTSGTRATSLGRPEEEEDELAHRCSSFMAPPVTDLGELRRRPGDMKTKMELLILETQAQVCQALAQVDGGANFSVDRWERKEGGGGISCVLQDGCVFEKAGVSISVVHGNLSEEAAKQMRSRGKVLKTKDGKLPFCAMGVSSVIHPKNPHAPTIHFNYRYFEVEEADGHKQWWFGGGCDLTPTYLNQEDAVHFHRTLKEACDQHGPDLYPKFKKWCDDYFFIAHRGERRGIGGIFFDDLDSPSKEEVFRFVQSCARAVVPSYIPLVKKHCDDSFTPQEKLWQQLRRGRYVEFNLLYDRGTKFGLFTPGSRIESILMSLPLTARWEYMHSPSENSKEAEILEVLRHPRDWVR

相同性解析による交差性が予測される生物種
Full Name coproporphyrinogen oxidase
Calculated molecular weight 454 aa, 50 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC017210
Gene Symbol CPOX
Gene ID (NCBI) 1371
RRIDAB_2084231
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP36551
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CPOX(Coproporphyrinogen-III oxidase, mitochondrial) is also named as CPO and CPX. Human CPO is a 76 kDa protein that is active as a homodimer in the mitochondrial intermembrane pace(PMID:16159891).Mutations in human CPO gene predict the clinical outcome of the disease, with either hepatic hereditary coproporphyria or hematological manifestations of erythropoietic harderoporphyria.CPOX has a transit peptide of 110 aa and the chain contains 344 aa.

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