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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CPNE3 Recombinant monoclonal antibody (83820-5-RR)

    WB analysis using 83820-5-RR (same clone as 83820-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83820-5-RR (CPNE3 antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83820-5-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A431 cells using CPNE3 Recombinant monoclonal antibody (83820-5-RR)

    WB analysis of A431 using 83820-5-RR (same clone as 83820-5-PBS)

    WB result of CPNE3 antibody (83820-5-RR; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CPNE3 transfected A431 cells. This data was developed using the same antibody clone with 83820-5-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HepG2 cells using CPNE3 Recombinant monoclonal antibody (83820-5-RR)

    IP experiment of HepG2 using 83820-5-RR (same clone as 83820-5-PBS)

    IP result of anti-CPNE3 (IP:83820-5-RR, 4ug; Detection:83820-5-RR 1:2000) with HepG2 cells lysate 1600 ug. This data was developed using the same antibody clone with 83820-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using CPNE3 Recombinant monoclonal antibody (83820-5-RR)

    IF Staining of A431 using 83820-5-RR (same clone as 83820-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CPNE3 antibody (83820-5-RR, Clone: 240871E5 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83820-5-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using CPNE3 Recombinant monoclonal antibody (83820-5-RR)

    FC experiment of HepG2 using 83820-5-RR (same clone as 83820-5-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug CPNE3 Recombinant antibody (83820-5-RR, Clone:240871E5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83820-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83820-5-RR

    Affinity and Kinetic Characterization of 83820-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83820-5-RR against Human CPNE3 were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO Validated

CPNE3 Recombinant monoclonal antibody, PBS Only

CPNE3 Uni-rAb® Recombinant Antibody for WB, IF/ICC, FC (Intra), IP, ELISA
Cat No. 83820-5-PBS
Clone No.240871E5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), IP, ELISA

Synonyms

240871E5, Copine 3, Copine III, Copine-3, copineIII

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83820-5-PBS targets CPNE3 in WB, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag1634

Product name: Recombinant human CPNE3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 238-537 aa of BC036242

Sequence: EASRSSPVEFECINEKKRQKKKSYKNSGVISVKQCEITVECTFLDYIMGGCQLNFTVGVDFTGSNGDPRSPDSLHYISPNGVNEYLTALWSVGLVIQDYDADKMFPAFGFGAQIPPQWQVSHEFPMNFNPSNPYCNGIQGIVEAYRSCLPQIKLYGQTNFSPIINHVARFAAAATQQQTASRYFVLLIITDGVITDLDETRQAIVNASRLPMSIIIVGVGGADFSAMEFLDGDGGSLHSPLGEVAIRDIVQFVPFRQFQNAPKEALAQCVLAEIPQQVVGYFNTYKLLPPKNPATKQQKQ

相同性解析による交差性が予測される生物種
Full Name copine III
Calculated molecular weight 60 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC036242
Gene Symbol CPNE3
Gene ID (NCBI) 8895
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDO75131
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Copines are a family of evolutionarily conserved calcium-dependent phospholipid-binding proteins (PMID: 9430674). They contain two Ca(2+)- and phospholipid-binding domains known as C2 domains. Copines are potentially involved in regulating membrane trafficking and in protein-protein interactions. Copine III (CPNE3) has also been identified as a ligand of ERBB2 and plays an important role in various carcinogenesis. The high expression of CPNE3 is an adverse prognostic biomarker for acute myeloid leukemia (PMID: 28670859).

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