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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CPNE3 Recombinant monoclonal antibody (83820-5-RR)

    WB analysis using 83820-5-RR (same clone as 83820-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83820-5-RR (CPNE3 antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83820-5-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A431 cells using CPNE3 Recombinant monoclonal antibody (83820-5-RR)

    WB analysis of A431 using 83820-5-RR (same clone as 83820-5-PBS)

    WB result of CPNE3 antibody (83820-5-RR; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CPNE3 transfected A431 cells. This data was developed using the same antibody clone with 83820-5-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HepG2 cells using CPNE3 Recombinant monoclonal antibody (83820-5-RR)

    IP experiment of HepG2 using 83820-5-RR (same clone as 83820-5-PBS)

    IP result of anti-CPNE3 (IP:83820-5-RR, 4ug; Detection:83820-5-RR 1:2000) with HepG2 cells lysate 1600 ug. This data was developed using the same antibody clone with 83820-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using CPNE3 Recombinant monoclonal antibody (83820-5-RR)

    IF Staining of A431 using 83820-5-RR (same clone as 83820-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CPNE3 antibody (83820-5-RR, Clone: 240871E5 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83820-5-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using CPNE3 Recombinant monoclonal antibody (83820-5-RR)

    FC experiment of HepG2 using 83820-5-RR (same clone as 83820-5-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug CPNE3 Recombinant antibody (83820-5-RR, Clone:240871E5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83820-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83820-5-RR

    Affinity and Kinetic Characterization of 83820-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83820-5-RR against Human CPNE3 were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO Validated

CPNE3 Recombinant monoclonal antibody, PBS Only

CPNE3 Uni-rAb® Recombinant Antibody for WB, IF/ICC, FC (Intra), IP, ELISA
Cat No. 83820-5-PBS
Clone No.240871E5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), IP, ELISA

Synonyms

240871E5, Copine 3, Copine III, Copine-3, copineIII

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83820-5-PBS targets CPNE3 in WB, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag1634

Product name: Recombinant human CPNE3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 238-537 aa of BC036242

Sequence: EASRSSPVEFECINEKKRQKKKSYKNSGVISVKQCEITVECTFLDYIMGGCQLNFTVGVDFTGSNGDPRSPDSLHYISPNGVNEYLTALWSVGLVIQDYDADKMFPAFGFGAQIPPQWQVSHEFPMNFNPSNPYCNGIQGIVEAYRSCLPQIKLYGQTNFSPIINHVARFAAAATQQQTASRYFVLLIITDGVITDLDETRQAIVNASRLPMSIIIVGVGGADFSAMEFLDGDGGSLHSPLGEVAIRDIVQFVPFRQFQNAPKEALAQCVLAEIPQQVVGYFNTYKLLPPKNPATKQQKQ

相同性解析による交差性が予測される生物種
Full Name copine III
Calculated molecular weight 60 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC036242
Gene Symbol CPNE3
Gene ID (NCBI) 8895
RRIDAB_3671405
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDO75131
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Copines are a family of evolutionarily conserved calcium-dependent phospholipid-binding proteins (PMID: 9430674). They contain two Ca(2+)- and phospholipid-binding domains known as C2 domains. Copines are potentially involved in regulating membrane trafficking and in protein-protein interactions. Copine III (CPNE3) has also been identified as a ligand of ERBB2 and plays an important role in various carcinogenesis. The high expression of CPNE3 is an adverse prognostic biomarker for acute myeloid leukemia (PMID: 28670859).

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