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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CPLX3 Polyclonal antibody (16949-1-AP)

    WB analysis using 16949-1-AP (same clone as 16949-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 16949-1-AP (CPLX3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16949-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using CPLX3 Polyclonal antibody (16949-1-AP)

    WB analysis of human brain using 16949-1-AP (same clone as 16949-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 16949-1-AP (CPLX3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16949-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using CPLX3 Polyclonal antibody (16949-1-AP)

    IP experiment of mouse brain using 16949-1-AP (same clone as 16949-1-PBS)

    IP result of anti-CPLX3 (IP:16949-1-AP, 3ug; Detection:16949-1-AP 1:300) with mouse brain tissue lysate 6000ug. This data was developed using the same antibody clone with 16949-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of rat eye tissue using CPLX3 Polyclonal antibody (16949-1-AP)

    IF Staining of rat eye using 16949-1-AP (same clone as 16949-1-PBS)

    Immunofluorescent analysis of (-20°C Acetone) fixed paraffin-embedded rat eye tissue using CPLX3 antibody (16949-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16949-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of rat eye tissue using CPLX3 Polyclonal antibody (16949-1-AP)

    IF Staining of rat eye using 16949-1-AP (same clone as 16949-1-PBS)

    Immunofluorescent analysis of (-20°C Acetone) fixed paraffin-embedded rat eye tissue using CPLX3 antibody (16949-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16949-1-PBS in a different storage buffer formulation.

CPLX3 Polyclonal antibody, PBS Only

CPLX3 Polyclonal Antibody for WB, IF-P, IP, Indirect ELISA
Cat No. 16949-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF-P, IP, Indirect ELISA

Synonyms

complexin 3, Complexin III, Complexin-3, CPX III, CPXIII

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16949-1-PBS targets CPLX3 in WB, IF-P, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag10571

Product name: Recombinant human CPLX3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-158 aa of BC018026

Sequence: MAFMVKTMVGGQLKNLTGSLGGGEDKGDGDKSAAEAQGMSREEYEEYQKQLVEEKMERDAQFTQRKAERATLRSHFRDKYRLPKNETDESQIQMAGGDVELPRELAKMIEEDTEEEEEKASVLGQLASLPGLNLGSLKDKAQATLGDLKQSAEKCHVM

相同性解析による交差性が予測される生物種
Full Name complexin 3
Calculated molecular weight 158 aa, 18 kDa
Observed molecular weight 20-23 kDa
GenBank accession numberBC018026
Gene Symbol CPLX3
Gene ID (NCBI) 594855
RRIDAB_2084042
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WVH0
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CPLX3, also named as Complexin-3, is 158 amino acid protein, which belongs to the complexin/synaphin family. CPLX3 localizes in the membrane and binds to the SNARE core complex containing SNAP25, VAMP2 and STX1A. CPLX3 positively regulates a late step in synaptic vesicle exocytosis.

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