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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using CPLX2 Monoclonal antibody (66849-1-Ig)

    WB analysis of mouse brain using 66849-1-Ig (same clone as 66849-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 66849-1-Ig (CPLX2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66849-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse cerebellum tissue using CPLX2 Monoclonal antibody (66849-1-Ig)

    WB analysis of mouse cerebellum using 66849-1-Ig (same clone as 66849-1-PBS)

    mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 66849-1-Ig (CPLX2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66849-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat brain tissue using CPLX2 Monoclonal antibody (66849-1-Ig)

    WB analysis of rat brain using 66849-1-Ig (same clone as 66849-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 66849-1-Ig (CPLX2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66849-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat cerebellum tissue using CPLX2 Monoclonal antibody (66849-1-Ig)

    WB analysis of rat cerebellum using 66849-1-Ig (same clone as 66849-1-PBS)

    rat cerebellum tissue were subjected to SDS PAGE followed by western blot with 66849-1-Ig (CPLX2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66849-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CPLX2 Monoclonal antibody (66849-1-Ig)

    IHC staining of mouse brain using 66849-1-Ig (same clone as 66849-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66849-1-Ig (CPLX2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66849-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CPLX2 Monoclonal antibody (66849-1-Ig)

    IHC staining of mouse brain using 66849-1-Ig (same clone as 66849-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66849-1-Ig (CPLX2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66849-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of SH-SY5Y cells using CPLX2 Monoclonal antibody (66849-1-Ig)

    FC experiment of SH-SY5Y using 66849-1-Ig (same clone as 66849-1-PBS)

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug CPLX2 Monoclonal antibody (66849-1-Ig, Clone:1B2A11) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66849-1-PBS in a different storage buffer formulation.

  • Cytometric bead array sample test of MP51525-1

    Cytometric bead array sample test of MP51525-1

    Cytometric bead array sample test of MP51525-1, CPLX2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66849-1-PBS. Detection antibody: 66849-2-PBS.

  • Cytometric bead array standard curve of MP51525-1

    Cytometric bead array standard curve of MP51525-1

    Cytometric bead array standard curve of MP51525-1, CPLX2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66849-1-PBS. Detection antibody: 66849-2-PBS. Standard:Ag27949. Range: 3.125-400 ng/mL.

CPLX2 Monoclonal antibody, PBS Only (Capture)

CPLX2 Monoclonal Antibody for WB, IHC, FC (Intra), Indirect ELISA
Cat No. 66849-1-PBS
Clone No.1B2A11

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, FC (Intra), Indirect ELISA

Synonyms

CPX 2, CPLX 2, Complexin-2, 921L, 921 L

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66849-1-PBS targets CPLX2 as part of a matched antibody pair:

MP51525-1: 66849-1-PBS capture and 66849-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag27949

Product name: Recombinant human CPLX2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-134 aa of BC093706

Sequence: MDFVMKQALGGATKDMGKMLGGEEEKDPDAQKKEEERQEALRQQEEERKAKHARMEAEREKVRQQIRDKYGLKKKEEKEAEEKAALEQPCEGSLTRPKKAIPAGCGDEEEEEEESILDTVLKYLPGPLQDMFKK

相同性解析による交差性が予測される生物種
Full Name complexin 2
Calculated molecular weight 134 aa, 15 kDa
Observed molecular weight 18-20 kDa
GenBank accession numberBC093706
Gene Symbol CPLX2
Gene ID (NCBI) 10814
RRIDAB_2882189
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDQ6PUV4
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Complexins are soluble proteins that regulate the activity of soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complexes necessary for vesicle fusion. Neuronal specific complexin 1 (CPLX1) has inhibitory and stimulatory effects on exocytosis by clamping trans-SNARE complexes in a prefusion state and promoting conformational changes to facilitate membrane fusion following cell stimulation. Complexin2 (CPLX2) is a pre-synaptic protein believed to regulate neurotransmitter release from pre-synaptic terminals, it is downregulated in schizophrenic patients suffering from depression, animal models of depression and neurological disorders such as Huntington's disease in which depression is a major symptom.

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