Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue, A549 cells, rat brain tissue |
| Positive IP detected in | A549 cells |
| Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 9 publications below |
| IHC | See 1 publications below |
| IF | See 2 publications below |
| IP | See 1 publications below |
Product Information
18149-1-AP targets CPLX2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12838 Product name: Recombinant human CPLX2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-134 aa of BC093706 Sequence: MDFVMKQALGGATKDMGKMLGGEEEKDPDAQKKEEERQEALRQQEEERKAKHARMEAEREKVRQQIRDKYGLKKKEEKEAEEKAALEQPCEGSLTRPKKAIPAGCGDEEEEEEESILDTVLKYLPGPLQDMFKK 相同性解析による交差性が予測される生物種 |
| Full Name | complexin 2 |
| Calculated molecular weight | 134 aa, 15 kDa |
| Observed molecular weight | 18-20 kDa |
| GenBank accession number | BC093706 |
| Gene Symbol | CPLX2 |
| Gene ID (NCBI) | 10814 |
| RRID | AB_2276580 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6PUV4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Complexins are soluble proteins that regulate the activity of soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complexes necessary for vesicle fusion. Neuronal specific complexin 1 (CPLX1) has inhibitory and stimulatory effects on exocytosis by clamping trans-SNARE complexes in a prefusion state and promoting conformational changes to facilitate membrane fusion following cell stimulation. Complexin2 (CPLX2) is a pre-synaptic protein believed to regulate neurotransmitter release from pre-synaptic terminals, it is downregulated in schizophrenic patients suffering from depression, animal models of depression and neurological disorders such as Huntington's disease in which depression is a major symptom.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CPLX2 antibody 18149-1-AP | Download protocol |
| IHC protocol for CPLX2 antibody 18149-1-AP | Download protocol |
| IP protocol for CPLX2 antibody 18149-1-AP | Download protocol |
| WB protocol for CPLX2 antibody 18149-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Acta Pharmacol Sin Complexin 2 contributes to the protective effect of NAD+ on neuronal survival following neonatal hypoxia-ischemia | ||
Life Sci Geniposide improves depression-like behavior in prenatal stress male offspring through restoring HPA axis- and glucocorticoid receptor-associated dysfunction | ||
Free Radic Biol Med NOX2 Deficiency Ameliorates Cerebral Injury by Reduction of Complexin II-mediated Glutamate Excitotoxicity in Experimental Stroke.
| ||
Behav Brain Res Widespread cerebellar transcriptome changes in Ts65Dn down syndrome mouse model after lifelong running. | ||
Cancer Biomark Complexin-2 (CPLX2) as a potential prognostic biomarker in human lung high grade neuroendocrine tumors. | ||
bioRxiv Role of Complexin 2 in the regulation of hormone secretion from the islet of Langerhans
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