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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Multi-rAb® CPE Multi-Recombinant antibody (RMX00074)

    WB analysis using RMX00074

    Various lysates were subjected to SDS PAGE followed by western blot with RMX00074 (CPE antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using Multi-rAb® CPE Multi-Recombinant antibody (RMX00074)

    IHC staining of mouse pancreas using RMX00074

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using RMX00074 (CPE antibody) at dilution of 1:100000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using Multi-rAb® CPE Multi-Recombinant antibody (RMX00074)

    IHC staining of mouse pancreas using RMX00074

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using RMX00074 (CPE antibody) at dilution of 1:100000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse pancreas tissue using Multi-rAb® CPE Multi-Recombinant antibody (RMX00074)

    IF Staining of mouse pancreas using RMX00074

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pancreas tissue using CPE antibody (RMX00074) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse pancreas tissue using Multi-rAb® CPE Multi-Recombinant antibody (RMX00074)

    IF Staining of mouse pancreas using RMX00074

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pancreas tissue using CPE antibody (RMX00074) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Multi-rAb® CPE Multi-Recombinant antibody

CPE Multi-Recombinant Antibody for WB, IHC, IF-P, ELISA
Cat No. RMX00074

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

Carboxypeptidase E, Carboxypeptidase H, CPH, EC:3.4.17.10, Prohormone-processing carboxypeptidase

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inMCF-7 cells, PC-12 cells, mouse brain tissue, rat brain tissue
Positive IHC detected inmouse pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse pancreas tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50000-1:200000
Immunofluorescence (IF)-PIF-P : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

RMX00074 targets CPE in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Multi-Recombinant
Type Antibody
Immunogen

FusionProtein

相同性解析による交差性が予測される生物種
Full Name carboxypeptidase E
Calculated molecular weight53 kDa
Observed molecular weight 50-55 kDa
GenBank accession numberNM_001873.4
Gene Symbol Carboxypeptidase E
Gene ID (NCBI) 1363
RRIDAB_3750123
Conjugate Unconjugated
Form
FormLiquid
Purification MethodN/A
UNIPROT IDP16870-1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CPE(Carboxypeptidase E) is also named as CPH.It belongs to the peptidase M14 family and is involved in the biosynthesis of peptide hormones and neurotransmitters, including insulin.The deduced 476-amino acid human protein contains an N-terminal signal peptide, followed by a 'pro' sequence, a polyarginine stretch, and 2 potential N-glycosylation sites.

Protocols

Product Specific Protocols
IF protocol for CPE antibody RMX00074Download protocol
IHC protocol for CPE antibody RMX00074Download protocol
WB protocol for CPE antibody RMX00074Download protocol
Standard Protocols
Click here to view our Standard Protocols
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