Validation Data Gallery

Filter: AllWBIHC

WB analysis of mouse pancreas using 12600-1-AP (same clone as 12600-1-PBS)
mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 12600-1-AP (CPB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12600-1-PBS in a different storage buffer formulation.
WB analysis of rat pancreas using 12600-1-AP (same clone as 12600-1-PBS)
rat pancreas tissue were subjected to SDS PAGE followed by western blot with 12600-1-AP (CPB1 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12600-1-PBS in a different storage buffer formulation.
WB analysis of mouse pancreas using 12600-1-AP (same clone as 12600-1-PBS)
mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 12600-1-AP (CPB1 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12600-1-PBS in a different storage buffer formulation.
WB analysis of mouse pancreas using 12600-1-AP (same clone as 12600-1-PBS)
mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 12600-1-AP (CPB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12600-1-PBS in a different storage buffer formulation.
IHC staining of human pancreas using 12600-1-AP (same clone as 12600-1-PBS)
Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 12600-1-AP (CPB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12600-1-PBS in a different storage buffer formulation.
IHC staining of human pancreas using 12600-1-AP (same clone as 12600-1-PBS)
Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 12600-1-AP (CPB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12600-1-PBS in a different storage buffer formulation.
IHC staining of human pancreas using 12600-1-AP (same clone as 12600-1-PBS)
Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 12600-1-AP (CPB1 antibody at dilution of 1:50. This data was developed using the same antibody clone with 12600-1-PBS in a different storage buffer formulation.
IHC staining of human pancreas using 12600-1-AP (same clone as 12600-1-PBS)
Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 12600-1-AP (CPB1 antibody at dilution of 1:50. This data was developed using the same antibody clone with 12600-1-PBS in a different storage buffer formulation.
IHC staining of human pancreas using 12600-1-AP (same clone as 12600-1-PBS)
Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 12600-1-AP (CPB1 antibody at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 12600-1-PBS in a different storage buffer formulation.
IHC staining of human pancreas using 12600-1-AP (same clone as 12600-1-PBS)
Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 12600-1-AP (CPB1 antibody at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 12600-1-PBS in a different storage buffer formulation.
IHC staining of human pancreas cancer using 12600-1-AP (same clone as 12600-1-PBS)
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12600-1-AP (CPB1 antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12600-1-PBS in a different storage buffer formulation.
IHC staining of human pancreas cancer using 12600-1-AP (same clone as 12600-1-PBS)
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12600-1-AP (CPB1 antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12600-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 12600-1-AP (same clone as 12600-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney using 12600-1-AP (CPB1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12600-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 12600-1-AP (same clone as 12600-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney using 12600-1-AP (CPB1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12600-1-PBS in a different storage buffer formulation.
IHC staining of human pancreas using 12600-1-AP (same clone as 12600-1-PBS)
Immunohistochemical analysis of paraffin-embedded human pancreas using 12600-1-AP (CPB1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12600-1-PBS in a different storage buffer formulation.
IHC staining of human pancreas using 12600-1-AP (same clone as 12600-1-PBS)
Immunohistochemical analysis of paraffin-embedded human pancreas using 12600-1-AP (CPB1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12600-1-PBS in a different storage buffer formulation.

CPB1 Polyclonal antibody, PBS Only

CPB1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 12600-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12600-1-PBS targets CPB1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag3243 Product name: Recombinant human PASP protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 73-417 aa of BC015338 Sequence: AEDTVTVENVLKQNELQYKVLISNLRNVVEAQFDSRVRATGHSYEKYNKWETIEAWTQQVATENPALISRSVIGTTFEGRAIYLLKVGKAGQNKPAIFMDCGFHAREWISPAFCQWFVREAVRTYGREIQVTELLDKLDFYVLPVLNIDGYIYTWTKSRFWRKTRSTHTGSSCIGTDPNRNFDAGWCEIGASRNPCDETYCGPAAESEKETKALADFIRNKLSSIKAYLTIHSYSQMMIYPYSYAYKLGENNAELNALAKATVKELASLHGTKYTYGPGATTIYPAAGGSDDWAYDQGIRYSFTFELRDTGRYGFLLPESQIRATCEETFLAIKYVASYVLEHLY 相同性解析による交差性が予測される生物種
Full Name	carboxypeptidase B1 (tissue)
Calculated molecular weight	417 aa, 47 kDa
Observed molecular weight	47 kDa
GenBank accession number	BC015338
Gene Symbol	CPB1
Gene ID (NCBI)	1360
RRID	AB_2261100
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P15086
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

Background Information

PASP(pancreas-specific protein) is also named as CPB1, CPB, PCPB and belongs to the peptidase M14 family. This protein modulates the plasminogen system both in the matrix and on the cell surface, thus leading to the inhibition of endothelial cell movement and tube formation. It is initially described as a pancreatic metallocarboxypeptidase and is a marker for acute pancreatitis(PMID:19717511). The deduced 416-amino acid preproenzyme has a 15-amino acid signal peptide and a 95-amino acid activation peptide.

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of mouse pancreas using 12600-1-AP (same clone as 12600-1-PBS)

WB analysis of rat pancreas using 12600-1-AP (same clone as 12600-1-PBS)

WB analysis of mouse pancreas using 12600-1-AP (same clone as 12600-1-PBS)

WB analysis of mouse pancreas using 12600-1-AP (same clone as 12600-1-PBS)

IHC staining of human pancreas using 12600-1-AP (same clone as 12600-1-PBS)

IHC staining of human pancreas using 12600-1-AP (same clone as 12600-1-PBS)

IHC staining of human pancreas using 12600-1-AP (same clone as 12600-1-PBS)

IHC staining of human pancreas using 12600-1-AP (same clone as 12600-1-PBS)

IHC staining of human pancreas using 12600-1-AP (same clone as 12600-1-PBS)

IHC staining of human pancreas using 12600-1-AP (same clone as 12600-1-PBS)

IHC staining of human pancreas cancer using 12600-1-AP (same clone as 12600-1-PBS)

IHC staining of human pancreas cancer using 12600-1-AP (same clone as 12600-1-PBS)

IHC staining of human kidney using 12600-1-AP (same clone as 12600-1-PBS)

IHC staining of human kidney using 12600-1-AP (same clone as 12600-1-PBS)

IHC staining of human pancreas using 12600-1-AP (same clone as 12600-1-PBS)

IHC staining of human pancreas using 12600-1-AP (same clone as 12600-1-PBS)

CPB1 Polyclonal antibody, PBS Only

CPB1 Polyclonal Antibody for WB, IHC, Indirect ELISA

-/ -

Tested Applications

Recommended dilution

Product Information

Background Information

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