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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Carboxypeptidase A5 Polyclonal antibody

Carboxypeptidase A5 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13731-1-AP

Synonyms

Carboxypeptidase A5, CPA5

Validation Data Gallery

Applications:
  • WB analysis of PC-3 using 13731-1-AP

    WB analysis of PC-3 using 13731-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 13731-1-AP (Carboxypeptidase A5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 13731-1-AP

    IHC staining of human testis using 13731-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 13731-1-AP (Carboxypeptidase A5 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human testis using 13731-1-AP

    IHC staining of human testis using 13731-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 13731-1-AP (Carboxypeptidase A5 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 13731-1-AP

    IHC staining of mouse testis using 13731-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13731-1-AP (Carboxypeptidase A5 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 13731-1-AP

    IHC staining of mouse testis using 13731-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13731-1-AP (Carboxypeptidase A5 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of PC-3 using 13731-1-AP

    IF Staining of PC-3 using 13731-1-AP

    Immunofluorescent analysis of PC-3 cells using 13731-1-AP (Carboxypeptidase A5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inPC-3 cells
Positive IHC detected inhuman testis tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

13731-1-AP targets Carboxypeptidase A5 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Carboxypeptidase A5 fusion protein Ag4755 相同性解析による交差性が予測される生物種
Full Name carboxypeptidase A5
Calculated molecular weight 436 aa, 49 kDa
Observed molecular weight 49 kDa
GenBank accession numberBC039362
Gene symbol CPA5
Gene ID (NCBI) 93979
RRIDAB_2261098
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Carboxypeptidase A5 antibody 13731-1-APDownload protocol
IHC protocol for Carboxypeptidase A5 antibody 13731-1-APDownload protocol
IF protocol for Carboxypeptidase A5 antibody 13731-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols