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Validation Data Gallery

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  • Western Blot (WB) analysis of PC-3 cells using Carboxypeptidase A5 Polyclonal antibody (13731-1-AP)

    WB analysis of PC-3 using 13731-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 13731-1-AP (Carboxypeptidase A5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using Carboxypeptidase A5 Polyclonal antibody (13731-1-AP)

    IHC staining of human testis using 13731-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 13731-1-AP (Carboxypeptidase A5 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human testis tissue using Carboxypeptidase A5 Polyclonal antibody (13731-1-AP)

    IHC staining of human testis using 13731-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 13731-1-AP (Carboxypeptidase A5 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using Carboxypeptidase A5 Polyclonal antibody (13731-1-AP)

    IHC staining of mouse testis using 13731-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13731-1-AP (Carboxypeptidase A5 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using Carboxypeptidase A5 Polyclonal antibody (13731-1-AP)

    IHC staining of mouse testis using 13731-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13731-1-AP (Carboxypeptidase A5 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using Carboxypeptidase A5 Polyclonal antibody (13731-1-AP)

    IF Staining of PC-3 using 13731-1-AP

    Immunofluorescent analysis of PC-3 cells using 13731-1-AP (Carboxypeptidase A5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Carboxypeptidase A5 Polyclonal antibody

Carboxypeptidase A5 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 13731-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

CPA5, EC:3.4.17.-

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inPC-3 cells
Positive IHC detected inhuman testis tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13731-1-AP targets Carboxypeptidase A5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag4755

Product name: Recombinant human CPA5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 126-436 aa of BC039362

Sequence: RLERSTNSFSYSSYHTLEEIYSWIDNFVMEHSDIVSKIQIGNSFENQSILVLKFSTGGSRHPAIWIDTGIHSREWITHATGIWTANKIVSDYGKDRVLTDILNAMDIFIELVTNPDGFAFTHSMNRLWRKNKSIRPGIFCIGVDLNRNWKSGFGGNGSNSNPCSETYHGPSPQSEPEVAAIVNFITAHGNFKALISIHSYSQMLMYPYGRLLEPVSNQRELYDLAKDAVEALYKVHGIEYIFGSISTTLYVASGITVDWAYDSGIKYAFSFELRDTGQYGFLLPATQIIPTAQETWMALRTIMEHTLNHPY

相同性解析による交差性が予測される生物種
Full Name carboxypeptidase A5
Calculated molecular weight 436 aa, 49 kDa
Observed molecular weight 49 kDa
GenBank accession numberBC039362
Gene Symbol CPA5
Gene ID (NCBI) 93979
RRIDAB_2261098
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WXQ8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Carboxypeptidase A5 (CPA5) is a member of the carboxypeptidase family. CPA5 was identified during a genome-wide search for human metallocarboxypeptidase-like genes with sequence similarity to other carboxypeptidases.

Protocols

Product Specific Protocols
IF protocol for Carboxypeptidase A5 antibody 13731-1-APDownload protocol
IHC protocol for Carboxypeptidase A5 antibody 13731-1-APDownload protocol
WB protocol for Carboxypeptidase A5 antibody 13731-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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