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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CPA1 Recombinant monoclonal antibody (88295-1-RR)

    WB analysis using 88295-1-RR (same clone as 88295-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 88295-1-RR (CPA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 88295-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CPA1 Recombinant monoclonal antibody (88295-1-RR)

    WB analysis using 88295-1-RR (same clone as 88295-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 88295-1-RR (CPA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 88295-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human pancreas tissue using CPA1 Recombinant monoclonal antibody (88295-1-RR)

    IF Staining of human pancreas using 88295-1-RR (same clone as 88295-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human pancreas tissue using CPA1 antibody (88295-1-RR, Clone: 260663D6 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 88295-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of rat pancreas tissue using CPA1 Recombinant monoclonal antibody (88295-1-RR)

    IF Staining of rat pancreas using 88295-1-RR (same clone as 88295-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat pancreas tissue using CPA1 antibody (88295-1-RR, Clone: 260663D6 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 88295-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of rat pancreas tissue using CPA1 Recombinant monoclonal antibody (88295-1-RR)

    IF Staining of rat pancreas using 88295-1-RR (same clone as 88295-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat pancreas tissue using CPA1 antibody (88295-1-RR, Clone: 260663D6 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 88295-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse pancreas tissue using CPA1 Recombinant monoclonal antibody (88295-1-RR)

    IF Staining of mouse pancreas using 88295-1-RR (same clone as 88295-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pancreas tissue using CPA1 antibody (88295-1-RR, Clone: 260663D6 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 88295-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse pancreas tissue using CPA1 Recombinant monoclonal antibody (88295-1-RR)

    IF Staining of mouse pancreas using 88295-1-RR (same clone as 88295-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pancreas tissue using CPA1 antibody (88295-1-RR, Clone: 260663D6 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 88295-1-PBS in a different storage buffer formulation.

CPA1 Recombinant monoclonal antibody, PBS Only

CPA1 Uni-rAb® Recombinant Antibody for WB, IF-P, Indirect ELISA
Cat No. 88295-1-PBS
Clone No.260663D6

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IF-P, Indirect ELISA

Synonyms

Carboxypeptidase A1, CPA, EC:3.4.17.1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

88295-1-PBS targets CPA1 in WB, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag8586

Product name: Recombinant human CPA1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 71-419 aa of BC005279

Sequence: SIQAVKIFLESHGISYETMIEDVQSLLDEEQEQMFAFRSRARSTDTFNYATYHTLEEIYDFLDLLVAENPHLVSKIQIGNTYEGRPIYVLKFSTGGSKRPAIWIDTGIHSREWVTQASGVWFAKKITQDYGQDAAFTAILDTLDIFLEIVTNPDGFAFTHSTNRMWRKTRSHTAGSLCIGVDPNRNWDAGFGLSGASSNPCSETYRGKFANSEVEVKSIVDFVKDHGNIKAFISIHSYSQLLMYPYGYKTEPVPDQDELDQLSKAAVTALASLYGTKFNYGSIIKAIYQASGSTIDWTYSQGIKYSFTFELRDTGRYGFLLPASQIIPTAKETWLALLTIMEHTLNHPY

相同性解析による交差性が予測される生物種
Full Name carboxypeptidase A1 (pancreatic)
Calculated molecular weight 419 aa, 47 kDa
Observed molecular weight 44-47 kDa
GenBank accession numberBC005279
Gene Symbol Carboxypeptidase A1
Gene ID (NCBI) 1357
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP15085
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CPA1(Carboxypeptidase A1) is also named as CPA and belongs to the the peptidase M14 family. CPA1 is one of the genes whose expression in the pancreas and the protein which it encodes is synthesized as an inactive precursor, proCPA1, which is processed to the active enzyme by the proteolytic removal of the 95-amino acid N-terminal prodomain. The mature CPA1 is not secreted and is trapped and degraded intracellularly(PMID:8639628). This antibody is speicific to CPA1.

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