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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CPA1 Monoclonal antibody (68826-1-Ig)

    WB analysis using 68826-1-Ig (same clone as 68826-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68826-1-Ig (CPA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68826-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse pancreas tissue using CPA1 Monoclonal antibody (68826-1-Ig)

    IF Staining of mouse pancreas using 68826-1-Ig (same clone as 68826-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pancreas tissue using CPA1 antibody (68826-1-Ig, Clone: 2H2E8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68826-1-PBS in a different storage buffer formulation.

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CPA1 Monoclonal antibody, PBS Only

CPA1 Monoclonal Antibody for WB, IF-P, ELISA
Cat No. 68826-1-PBS
Clone No.2H2E8

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IF-P, ELISA

Synonyms

Carboxypeptidase A1, EC:3.4.17.1, CPA, 2H2E8

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68826-1-PBS targets CPA1 in WB, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CPA1 fusion protein Ag8579 相同性解析による交差性が予測される生物種
Full Name carboxypeptidase A1 (pancreatic)
Calculated molecular weight 419 aa, 47 kDa
Observed molecular weight47 kDa
GenBank accession numberBC005279
Gene Symbol Carboxypeptidase A1
Gene ID (NCBI) 1357
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP15085
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

CPA1(Carboxypeptidase A1) is also named as CPA and belongs to the the peptidase M14 family. CPA1 is one of the genes whose expression in the pancreas and the protein which it encodes is synthesized as an inactive precursor, proCPA1, which is processed to the active enzyme by the proteolytic removal of the 95-amino acid N-terminal prodomain. The mature CPA1 is not secreted and is trapped and degraded intracellularly(PMID:8639628). This antibody is speicific to CPA1.