Validation Data Gallery
Tested Applications
| Positive WB detected in | human blood tissue, mouse brain tissue, human plasma tissue |
| Positive IHC detected in | human tonsillitis tissue, human colon tissue, human hepatocirrhosis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 9 publications below |
| IHC | See 4 publications below |
| IF | See 2 publications below |
Product Information
21131-1-AP targets Ceruloplasmin in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, chicken |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag15535 Product name: Recombinant human CP protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 194-446 aa of BC146663 Sequence: IGPLIICKKDSLDKEKEKHIDREFVVMFSVVDENFSWYLEDNIKTYCSEPEKVDKDNEDFQESNRMYSVNGYTFGSLPGLSMCAEDRVKWYLFGMGNEVDVHAAFFHGQALTNKNYRIDTINLFPATLFDAYMVAQNPGEWMLSCQNLNHLKAGLQAFFQVQECNKSSSKDNIRGKHVRHYYIAAEEIIWNYAPSGIDIFTKENLTAPGSDSAVFFEQGTTRIGGSYKKLVYREYTDASFTNRKERGPEEEHL 相同性解析による交差性が予測される生物種 |
| Full Name | ceruloplasmin (ferroxidase) |
| Calculated molecular weight | 1065 aa, 122 kDa |
| Observed molecular weight | 130-140 kDa, 85 kDa |
| GenBank accession number | BC146663 |
| Gene Symbol | Ceruloplasmin |
| Gene ID (NCBI) | 1356 |
| RRID | AB_2878815 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P00450 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Ceruloplasmin (CP) is a serum glycoprotein synthesized by hepatocytes and functions as the major transport protein of copper in blood where it acts as ferrodoxidase and is required for iron transport of transferrin. The intact ceruloplasmin migrated around 130-140 kDa and some proteolytic cleaved fragments could also be observed.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Ceruloplasmin antibody 21131-1-AP | Download protocol |
| WB protocol for Ceruloplasmin antibody 21131-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Int J Biol Sci Novel LncRNA LINC02936 Suppresses Ferroptosis and Promotes Tumor Progression by Interacting with SIX1/CP Axis in Endometrial Cancer
| ||
Carcinogenesis Identification of HCAR1 as a ferroptosis-related biomarker of gastric cancer based on a novel ferroptosis-related prognostic model and in vitro experiments | ||
J Clin Pharmacol Expression of ceruloplasmin in the peripheral blood of patients with drug-resistant epilepsy | ||
Ecotoxicol Environ Saf Bioinformatics-based dynamics of cuproptosis -related indicators in experimental silicosis | ||
Cell Death Dis The divergent effects of astrocyte ceruloplasmin on learning and memory function in young and old mice | ||
iScience Hepatic depletion of nucleolar protein mDEF causes excessive mitochondrial copper accumulation associated with p53 and NRF1 activation |