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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using COXIV Recombinant antibody (82916-1-RR)

    WB analysis of HepG2 using 82916-1-RR

    WB result of COXIV antibody (82916-1-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-COXIV transfected HepG2 cells.

  • Western Blot (WB) analysis of various lysates using COXIV Recombinant antibody (82916-1-RR)

    WB analysis using 82916-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82916-1-RR (COXIV antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using COXIV Recombinant antibody (82916-1-RR)

    WB analysis using 82916-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82916-1-RR (COXIV antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using COXIV Recombinant antibody (82916-1-RR)

    WB analysis using 82916-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82916-1-RR (COXIV antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach tissue using COXIV Recombinant antibody (82916-1-RR)

    IHC staining of human stomach using 82916-1-RR

    Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 82916-1-RR (COXIV antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using COXIV Recombinant antibody (82916-1-RR)

    IHC staining of human colon using 82916-1-RR

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 82916-1-RR (COXIV antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovarian cancer using COXIV Recombinant antibody (82916-1-RR)

    IHC staining of human ovarian cancer using 82916-1-RR

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 82916-1-RR (COXIV antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using COXIV Recombinant antibody (82916-1-RR)

    IF Staining of HepG2 using 82916-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using COXIV antibody (82916-1-RR, Clone: 3K20 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of HeLa cells using COXIV Recombinant antibody (82916-1-RR)

    FC experiment of HeLa using 82916-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug COXIV Recombinant antibody (82916-1-RR, Clone:3K20) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • Affinity and Kinetic Characterization of 82916-1-RR

    Affinity and Kinetic Characterization of 82916-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82916-1-RR against Human COXIV were performed. The affinity constant is 9.41 nM.

  • Featured Product
  • KD/KO Validated

COXIV Recombinant monoclonal antibody

COXIV Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 82916-1-RR
Clone No.3K20

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

COX4I1, 3K20, COX IV-1, COX4, Cytochrome c oxidase subunit 4 isoform 1, mitochondrial

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, mouse skeletal muscle tissue, Jurkat cells, A431 cells, rat skeletal muscle tissue, mouse liver tissue
Positive IHC detected inhuman stomach tissue, human colon tissue, human ovarian cancer
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:600-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82916-1-RR targets COXIV in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag1640

Product name: Recombinant human COXIV protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-169 aa of BC021236

Sequence: MLATRVFSLVGKRAISTSVCVRAHESVVKSEDFSLPAYMDRRDHPLPEVAHVKHLSASQKALKEKEKASWSSLSMDEKVELYRIKFKESFAEMNRGSNEWKTVVGGAMFFIGFTALVIMWQKHYVYGPLPQSFDKEWVAKQTKRMLDMKVNPIQGLASKWDYEKNEWKK

相同性解析による交差性が予測される生物種
Full Name cytochrome c oxidase subunit IV isoform 1
Calculated molecular weight19.6 kDa
Observed molecular weight17-18 kDa
GenBank accession numberBC021236
Gene Symbol COX IV
Gene ID (NCBI) 1327
RRIDAB_3670649
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP13073
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

COX4I1, also named as COX4 and COXIV-1, belongs to the cytochrome c oxidase IV family. It is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. COX4I1 is a marker for mitochondria. It has two isoforms (isoform 1 and 2). Isoform 1(COX4I1) is ubiquitously expressed and isoform 2 is highly expressed in lung tissues.

Protocols

Product Specific Protocols
FC protocol for COXIV antibody 82916-1-RRDownload protocol
IF protocol for COXIV antibody 82916-1-RRDownload protocol
IHC protocol for COXIV antibody 82916-1-RRDownload protocol
WB protocol for COXIV antibody 82916-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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