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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse skeletal muscle tissue using COX8A Polyclonal antibody (15368-1-AP)

    WB analysis of mouse skeletal muscle using 15368-1-AP (same clone as 15368-1-PBS)

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15368-1-AP (COX8A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15368-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human skeletal muscle tissue using COX8A Polyclonal antibody (15368-1-AP)

    WB analysis of human skeletal muscle using 15368-1-AP (same clone as 15368-1-PBS)

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15368-1-AP (COX8A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15368-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat heart tissue using COX8A Polyclonal antibody (15368-1-AP)

    IHC staining of rat heart using 15368-1-AP (same clone as 15368-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 15368-1-AP (COX8A antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15368-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat heart tissue using COX8A Polyclonal antibody (15368-1-AP)

    IHC staining of rat heart using 15368-1-AP (same clone as 15368-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 15368-1-AP (COX8A antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15368-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using COX8A Polyclonal antibody (15368-1-AP)

    IHC staining of mouse heart using 15368-1-AP (same clone as 15368-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15368-1-AP (COX8A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15368-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using COX8A Polyclonal antibody (15368-1-AP)

    IHC staining of human hepatocirrhosis using 15368-1-AP (same clone as 15368-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 15368-1-AP (COX8A antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 15368-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using COX8A Polyclonal antibody (15368-1-AP)

    IHC staining of human hepatocirrhosis using 15368-1-AP (same clone as 15368-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 15368-1-AP (COX8A antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 15368-1-PBS in a different storage buffer formulation.

COX8A Polyclonal antibody, PBS Only

COX8A Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 15368-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

COX8, COX8 2, COX8L, Cytochrome c oxidase polypeptide VIII-liver/heart, Cytochrome c oxidase subunit 8-2

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15368-1-PBS targets COX8A in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag2885

Product name: Recombinant human COX8A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-69 aa of BC063025

Sequence: MSVLTPLLLRGLTGSARRLPVPRAKIHSLPPEGKLGIMELAVGLTSCFVTFLLPAGWILSHLETYRRPE

相同性解析による交差性が予測される生物種
Full Name cytochrome c oxidase subunit 8A (ubiquitous)
Calculated molecular weight 8 kDa
Observed molecular weight 8 kDa
GenBank accession numberBC063025
Gene Symbol COX8A
Gene ID (NCBI) 1351
RRIDAB_10697832
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP10176
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

COX8A(Cytochrome c oxidase subunit 8A) is also named as COX8, COX8L and belongs to the cytochrome c oxidase VIII family. This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. The gene encodes a full length protein of 8 kDa with a transit peptide of 25 amino acid.

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