Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue, HeLa cells, rat brain tissue, SH-SY5Y cells |
| Positive IHC detected in | human colon tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:5000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 21 publications below |
| IHC | See 3 publications below |
Product Information
11448-1-AP targets COX5A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, monkey, zebrafish, chimpanzee |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2005 Product name: Recombinant human COX5A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-150 aa of BC024240 Sequence: MLGAALRRCAVAATTRADPRGLLHSARTPGPAVAIQSVRCYSHGSQETDEEFDARWVTYFNKPDIDAWELRKGINTLVTYDMVPEPKIIDAALRACRRLNDFASTVRILEVVKDKAGPHKEIYPYVIQELRPTLNELGISTPEELGLDKV 相同性解析による交差性が予測される生物種 |
| Full Name | cytochrome c oxidase subunit Va |
| Calculated molecular weight | 150 aa, 17 kDa |
| Observed molecular weight | 13 kDa |
| GenBank accession number | BC024240 |
| Gene Symbol | COX5A |
| Gene ID (NCBI) | 9377 |
| RRID | AB_2085429 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P20674 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
COX5A(Cytochrome c oxidase subunit 5A, mitochondrial)is also named COXVA and belongs to the cytochrome c oxidase subunit 5A family. This is the heme A-containing chain of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. The mature human COX Va polypeptide shares 35% and 95% protein sequence identity with the corresponding S. cerevisiae COX subunit VI and bovine COX Va, respectively(PMID:2853101).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for COX5A antibody 11448-1-AP | Download protocol |
| IHC protocol for COX5A antibody 11448-1-AP | Download protocol |
| WB protocol for COX5A antibody 11448-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cancer Res CK1δ/CK1ε Signaling Sustains Mitochondrial Metabolism and Cell Survival in Multiple Myeloma | ||
Nucleic Acids Res Deletion of Gtpbp3 in zebrafish revealed the hypertrophic cardiomyopathy manifested by aberrant mitochondrial tRNA metabolism. | ||
Nucleic Acids Res Ablation of Mto1 in zebrafish exhibited hypertrophic cardiomyopathy manifested by mitochondrion RNA maturation deficiency. | ||
J Biomed Sci Optimized allotopic expression of mitochondrial ND6 transgene restored complex I and apoptosis deficiencies caused by LHON-linked ND6 14484T > C mutation | ||
Adv Sci (Weinh) Aloe Emodin Alleviates Radiation-Induced Heart Disease via Blocking P4HB Lactylation and Mitigating Kynurenine Metabolic Disruption | ||
Cell Death Dis Bromodomain-containing protein 4 (BRD4) as an epigenetic regulator of fatty acid metabolism genes and ferroptosis |